Molecular, biochemical and immunological tumor diagnosis

This project is based on investigation of gene alterations, disturbance in intracellular signal pathways simultaneously with investigations of diversity in immunophenotype in different cancer including leukemia, lymphoma, breast cancer, lung cancer, colon cancer and odontogenic tumor in maxillofacial region. Investigation of tumor suppressor gene (p53) and Ras mutation, p16INK4ACpG that have important role in epigenetic regulation will be first part of research. Investigation of LDH, their isoenzymes, mechanism of their regulation trough cell membrane in separated cells from cancer patients will be second topic of investigation in our project. We also have plan to investigated oxidative stress enzymes in different cancer in respect to molecules involved in regulation of apoptosis (bcl-2 and bax). Immunophenotype will be focused on investigation of expression of several molecules involved in TNF receptor superfamily (TNFR1, TNFR2, TGF-transforming growth factor, FADD, CD95, CD45, CD30) on tumor cells simultaneously with markers for cell proliferation (Ki-67, CD23, CD38, CD14, CD25, CD31-PECAM) in patients with leukemia and lung cancer. The hormonal receptors (Herceptin/Her-2 receptor, Estrogen, progesteron receptors) will be analyzed in breast cancer patients while E-Cadherin in odontogenic tumors. Degree of neovascularisation will be analyzed by presence of VEGF-vascular endothelial growth factor, ?GF-epidermal growth factor and hypoxia inducible factors, HIF-1 expression.