Projects / Programmes source: ARRS

Molecular markers and transformation techniques in crops

Research activity

Code Science Field Subfield
4.03.01  Biotechnical sciences  Plant production  Agricultural plants 

Code Science Field
B225  Biomedical sciences  Plant genetics 
B390  Biomedical sciences  Phytotechny, horticulture, crop protection, phytopathology 
molecular markers, genetic variability, scab resistance, transformation, hop, apple, buckwheat
Evaluation (rules)
source: COBISS
Researchers (3)
no. Code Name and surname Research area Role Period No. of publications
1.  09565  PhD Borut Bohanec  Biotechnology  Researcher  1998 - 2001  592 
2.  05994  PhD Branka Javornik  Plant production  Principal Researcher  1998 - 2001  1,282 
3.  05083  PhD Zlata Luthar  Biotechnology  Researcher  1998 - 2001  349 
Project includes genetic studies in hop (Humulus lupulus L.) and apple (Malus spp.) by means of molecular markers in order to evaluate genetic characteristic important for breeding and an introduction of indirect gene transfer technique via Agrobacterium tumefaciens. In hop, an extensive collection of around 65 accessions from the gene bank will be analysed by means of molecular markers for the evaluation of their genetic variability and relationships to obtain important parameters for development of new cultivars. In apple, 28 scab-resistant cultivars, four wild source of the resistance genes and 10 susceptible cultivars will be screened for the presence of the RAPD markers, which were reported to be linked to the scab resistance gene Vf. RAPD analysis will also be employed for the evaluation of genetic stability in somaclones raised in vitro. The purpose of the work with gene transfer will be to elaborate technique with Agrobacterium tumefaciens. Work will be conducted on buckwheat (Fagopyrum esculentum Moench) where we have developed sufficient level or regeneration and in which we could, in continuation, introduce economically important genes.
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