Loading...
Projects / Programmes source: ARIS

The impact of acute hyperglycaemia on the activation of cognitive areas in the brain, on oxidative stress related gene expression and on epigenetic markers

Research activity

Code Science Field Subfield
3.05.00  Medical sciences  Human reproduction   

Code Science Field
B007  Biomedical sciences  Medicine (human and vertebrates) 

Code Science Field
3.02  Medical and Health Sciences  Clinical medicine 
Keywords
acute hyperglycaemia, brain damage, cognitive function, chronic complications, inflammation, oxidative stress, ncRNA profiling, functional MRI
Evaluation (rules)
source: COBISS
Researchers (19)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  13023  PhD Tadej Battelino  Medical sciences  Head  2018 - 2020  1,246 
2.  13409  PhD Nataša Bratina  Human reproduction  Researcher  2018 - 2020  433 
3.  27580  PhD Klara Bulc Rozman  Neurobiology  Researcher  2018 - 2020  19 
4.  15657  PhD Maruša Debeljak  Oncology  Researcher  2018 - 2020  248 
5.  34849  PhD Klemen Dovč  Metabolic and hormonal disorders  Researcher  2018 - 2020  163 
6.  35087  Ana Gianini    Technical associate  2018 - 2020 
7.  28512  PhD Tinka Hovnik  Medical sciences  Researcher  2018 - 2020  107 
8.  15169  PhD Damijana Mojca Jurič  Neurobiology  Researcher  2018 - 2020  103 
9.  21358  PhD Primož Kotnik  Human reproduction  Researcher  2018 - 2020  247 
10.  32181  PhD Jernej Kovač  Medical sciences  Researcher  2018 - 2020  210 
11.  29097  Brigita Mali    Technical associate  2018 - 2020 
12.  35089  Barbara Murn Berkopec    Technical associate  2018 - 2020  18 
13.  37426  PhD Robert Šket  Human reproduction  Researcher  2019 - 2020  72 
14.  34103  PhD Darja Šmigoc Schweiger  Human reproduction  Researcher  2018 - 2020  40 
15.  07002  PhD Dušan Šuput  Neurobiology  Researcher  2018 - 2020  434 
16.  50622  PhD Urša Šuštar  Metabolic and hormonal disorders  Junior researcher  2018 - 2020  31 
17.  37490  PhD Tine Tesovnik  Human reproduction  Researcher  2018 - 2020  65 
18.  20253  PhD Katarina Trebušak Podkrajšek  Human reproduction  Researcher  2018 - 2020  407 
19.  26268  PhD Andrej Vovk  Neurobiology  Researcher  2018 - 2019  53 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0312  University Medical Centre Ljubljana  Ljubljana  5057272000  77,757 
2.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,536 
Abstract
T1D is one of the most common chronic diseases of the childhood. It is caused by autoimmune destruction of pancreatic β-cells that produce insulin. Due to the lack of insulin secretion from pancreatic β-cells energy metabolism is disrupted. Insulin, the key anabolic hormone in the body, enables the uptake of glucose from the blood into the cells. Catabolic state and elevated blood glucose (hyperglycaemia) are the consequences of insulin shortage. According to the latest published data, the incidence of type 1 diabetes mellitus in Slovenia during 1990–2005 in children aged between 0–14 years is 11,67/100.000.19 Every year approximately 60 new cases are reported. The incidence of T1D is increasing worldwide by 3-5% per year. There is a lot of information about the influence of hypoglycaemia on the function of central nervous system (CNS), but the information regarding the influence of hyperglycaemia on CNS in scarce. As hyperglycaemia is a common acute complication, it is of utmost importance to investigate its short- and long-term influence on CNS. T1D is one of the most common chronic diseases of the childhood. It is caused by autoimmune destruction of pancreatic β - cells that produce insulin. Due to the lack of insulin secretion from pancreatic β - cells energy metabolism is disrupted. Insulin, the key anabolic hormone in the body, enables the uptake of glucose from the blood into the cells. Catabolic state and elevated blood glucose (hyperglycaemia) are the consequences of insulin shortage. Classic symptoms of hyperglycaemia in T1D are: polyuria, polydipsia, polyfagia and weight loss in some children, acidosis is also present. According to the latest published data, the incidence of type 1 diabetes mellitus in Slovenia during 1990 – 2005 in children aged between 0 – 14 years is 11,67/100.000. Every year approximately 60 new cases are reported. The incidence of T1D is increasing worldwide by 3 - 5% per year. There is a lot of information about the influence of hypoglycaemia on the function of central nervous system (CNS), but the information regarding the influence of hyperglycaemia on  CNS in scarce. As hyperglycaemia is a common acute complication, it is of utmost importance to investigate its short- and long- term influence on CNS.  We are going to focus on the influence of 120 – minute hyperglycaemia on the activation of cognitive areas in the brain, involved in planning, spatial memory and impulsiveness, by performing fMRI. We are also going to study the influence of 120 – minute hyperglycaemia on concentration of neurotransmitters (glucose, lactate, myoinositol) in the selected areas of the brain (frontal cortex), by performing 1 H-MRS in normoglycaemic and hyperglycaemic state. We are going to study the influence of 120 - minute hyperglycaemia (blood glucose 20 mmol/l) on oxidative stress (nitrotyrozine) and inflammation (IL-6, fibrinogen) andexpression of ncRNA in children and adolescents with T1D; So far, no publication/article describing fMRI during hyperglycaemia is available, which is why the results of our research are going to be completely original. We expect that more cognitive areas in the brain are going to be activated during planning and spatial memory tasks preformed in acute hyperglycaemia as compared to euglycaemic state. We also expect to see the change in neurotransmitter levels in certain areas of the brain during acute hyperglycaemia, marking the change in signal transmission, increased cell membrane disintegration and dysfunction. We expect to confirm the influence of acute hyperglycaemia on increased inflammation and oxidative stress markers. We also expect to prove the change of ncRNA expression and tissue specific exones in plasma. The exact cellular mechanisms of release of the ncRNA from the cells are not yet specified. However ncRNA is an interesting biomarker due to its stability. It can be detected via PCR; the detection is very sensitive and specific. In future, ncRNA could be marker of
Significance for science
So far no publication/article describing fMRI during hyperglycaemia is available, which is why the results of our research are going to e completely original. We expect that more cognitive areas in the brain are going to be activated during planning and spatial memory tasks preformed in acute hyperglycaemia, marking the change in signal trnsmission, increased cell membrane disintegration and dysfunction. We expect to confirm the influence of acute hyperglycaemia on increased inflammation and oxidative stress markers.  we also expect to prove the change of ncRNA expression and tissue specific exones in plasma. the exact cellular mechanisms of release of the ncRNA from the cells are not yet specified. However, ncRNA is an interseting biomarker due to its stability. It can be detected via PCR; the detection is verysensitive and specific. In future ncRNA could be marker of success of type 1 diabetes control.  Future research is likely going to be directed toward discovery of effective antioxidant drug. Results of this study will provide data for clinical guidelines on cognitive functioning during hyperglycemia in type 1 diabetes, relevant in all school children and during tasks requiring cognitive functions (e.g. driving). As currently no data on cognitive functioning with fMRI during hyperglycaemia are available, this study will provide essential new data relevant for millions of youth with type 1 diabetes throughout the world.
Significance for the country
So far no publication/article describing fMRI during hyperglycaemia is available, which is why the results of our research are going to e completely original. We expect that more cognitive areas in the brain are going to be activated during planning and spatial memory tasks preformed in acute hyperglycaemia, marking the change in signal trnsmission, increased cell membrane disintegration and dysfunction. We expect to confirm the influence of acute hyperglycaemia on increased inflammation and oxidative stress markers.  we also expect to prove the change of ncRNA expression and tissue specific exones in plasma. the exact cellular mechanisms of release of the ncRNA from the cells are not yet specified. However, ncRNA is an interseting biomarker due to its stability. It can be detected via PCR; the detection is verysensitive and specific. In future ncRNA could be marker of success of type 1 diabetes control.  Future research is likely going to be directed toward discovery of effective antioxidant drug. Results of this study will provide data for clinical guidelines on cognitive functioning during hyperglycemia in type 1 diabetes, relevant in all school children and during tasks requiring cognitive functions (e.g. driving). As currently no data on cognitive functioning with fMRI during hyperglycaemia are available, this study will provide essential new data relevant for millions of youth with type 1 diabetes throughout the world.
Most important scientific results Interim report, final report
Most important socioeconomically and culturally relevant results Final report
Views history
Favourite