Projects / Programmes
The impact of acute hyperglycaemia on the activation of cognitive areas in the brain, on oxidative stress related gene expression and on epigenetic markers
| Code |
Science |
Field |
Subfield |
| 3.05.00 |
Medical sciences |
Human reproduction |
|
| Code |
Science |
Field |
| B007 |
Biomedical sciences |
Medicine (human and vertebrates) |
| Code |
Science |
Field |
| 3.02 |
Medical and Health Sciences |
Clinical medicine |
acute hyperglycaemia, brain damage, cognitive function, chronic complications, inflammation, oxidative stress, ncRNA profiling, functional MRI
Researchers (19)
Organisations (2)
Abstract
T1D is one of the most common chronic diseases of the childhood. It is caused by autoimmune destruction of pancreatic β-cells that produce insulin. Due to the lack of insulin secretion from pancreatic β-cells energy metabolism is disrupted. Insulin, the key anabolic hormone in the body, enables the uptake of glucose from the blood into the cells. Catabolic state and elevated blood glucose (hyperglycaemia) are the consequences of insulin shortage. According to the latest published data, the incidence of type 1 diabetes mellitus in Slovenia during 1990–2005 in children aged between 0–14 years is 11,67/100.000.19 Every year approximately 60 new cases are reported. The incidence of T1D is increasing worldwide by 3-5% per year. There is a lot of information about the influence of hypoglycaemia on the function of central nervous system (CNS), but the information regarding the influence of hyperglycaemia on CNS in scarce. As hyperglycaemia is a common acute complication, it is of utmost importance to investigate its short- and long-term influence on CNS.
T1D is one of the most common chronic diseases of the childhood. It is caused by autoimmune destruction of pancreatic β - cells that produce insulin. Due to the lack of insulin secretion from pancreatic β - cells energy metabolism is disrupted. Insulin, the key anabolic hormone in the body, enables the uptake of glucose from the blood into the cells.
Catabolic state and elevated blood glucose (hyperglycaemia) are the consequences of insulin shortage.
Classic symptoms of hyperglycaemia in T1D are: polyuria, polydipsia, polyfagia and weight loss in some children, acidosis is also present. According to the latest published data, the incidence of type 1 diabetes mellitus in Slovenia during 1990 – 2005 in children aged between 0 – 14 years is 11,67/100.000. Every year approximately 60 new cases are reported. The incidence of T1D is increasing worldwide by 3 - 5% per year.
There is a lot of information about the influence of hypoglycaemia on the function of central
nervous system (CNS), but the information regarding the influence of hyperglycaemia on
CNS in scarce. As hyperglycaemia is a common acute complication, it is of utmost importance to investigate its short- and long- term influence on CNS.
We are going to focus on the influence of 120 – minute hyperglycaemia on the activation of cognitive areas in the brain, involved in planning, spatial memory and impulsiveness, by performing fMRI. We are also going to study the influence of 120 – minute hyperglycaemia on concentration of neurotransmitters (glucose, lactate, myoinositol) in the selected areas of the brain (frontal cortex), by performing 1 H-MRS in normoglycaemic and hyperglycaemic state. We are going to study the influence of 120 - minute hyperglycaemia (blood glucose 20 mmol/l) on oxidative stress (nitrotyrozine) and inflammation (IL-6, fibrinogen) andexpression of ncRNA in children and adolescents with T1D;
So far, no publication/article describing fMRI during hyperglycaemia is available, which is
why the results of our research are going to be completely original.
We expect that more cognitive areas in the brain are going to be activated during planning
and spatial memory tasks preformed in acute hyperglycaemia as compared to euglycaemic
state. We also expect to see the change in neurotransmitter levels in certain areas of the
brain during acute hyperglycaemia, marking the change in signal transmission, increased
cell membrane disintegration and dysfunction.
We expect to confirm the influence of acute hyperglycaemia on increased inflammation and
oxidative stress markers.
We also expect to prove the change of ncRNA expression and tissue specific exones in
plasma. The exact cellular mechanisms of release of the ncRNA from the cells are not yet
specified. However ncRNA is an interesting biomarker due to its stability. It can be detected
via PCR; the detection is very sensitive and specific. In future, ncRNA could be marker of
Significance for science
So far no publication/article describing fMRI during hyperglycaemia is available, which is why the results of our research are going to e completely original.
We expect that more cognitive areas in the brain are going to be activated during planning and spatial memory tasks preformed in acute hyperglycaemia, marking the change in signal trnsmission, increased cell membrane disintegration and dysfunction.
We expect to confirm the influence of acute hyperglycaemia on increased inflammation and oxidative stress markers.
we also expect to prove the change of ncRNA expression and tissue specific exones in plasma. the exact cellular mechanisms of release of the ncRNA from the cells are not yet specified. However, ncRNA is an interseting biomarker due to its stability. It can be detected via PCR; the detection is verysensitive and specific. In future ncRNA could be marker of success of type 1 diabetes control.
Future research is likely going to be directed toward discovery of effective antioxidant drug.
Results of this study will provide data for clinical guidelines on cognitive functioning during hyperglycemia in type 1 diabetes, relevant in all school children and during tasks requiring cognitive functions (e.g. driving). As currently no data on cognitive functioning with fMRI during hyperglycaemia are available, this study will provide essential new data relevant for millions of youth with type 1 diabetes throughout the world.
Significance for the country
So far no publication/article describing fMRI during hyperglycaemia is available, which is why the results of our research are going to e completely original.
We expect that more cognitive areas in the brain are going to be activated during planning and spatial memory tasks preformed in acute hyperglycaemia, marking the change in signal trnsmission, increased cell membrane disintegration and dysfunction.
We expect to confirm the influence of acute hyperglycaemia on increased inflammation and oxidative stress markers.
we also expect to prove the change of ncRNA expression and tissue specific exones in plasma. the exact cellular mechanisms of release of the ncRNA from the cells are not yet specified. However, ncRNA is an interseting biomarker due to its stability. It can be detected via PCR; the detection is verysensitive and specific. In future ncRNA could be marker of success of type 1 diabetes control.
Future research is likely going to be directed toward discovery of effective antioxidant drug.
Results of this study will provide data for clinical guidelines on cognitive functioning during hyperglycemia in type 1 diabetes, relevant in all school children and during tasks requiring cognitive functions (e.g. driving). As currently no data on cognitive functioning with fMRI during hyperglycaemia are available, this study will provide essential new data relevant for millions of youth with type 1 diabetes throughout the world.
Most important scientific results
Interim report,
final report
Most important socioeconomically and culturally relevant results
Final report
