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Projects / Programmes source: ARIS

Identifying the genetic determinants of chemical toxicity in the green alga Chlamydomonas reinhardtii

Research activity

Code Science Field Subfield
4.06.05  Biotechnical sciences  Biotechnology  Plant biotechnology 

Code Science Field
2.08  Engineering and Technology  Environmental biotechnology  
Keywords
functional genomics, ecotoxicology, adverse outcome pathways, chlamydomonas
Evaluation (rules)
source: COBISS
Points
9,182.55
A''
2,990.92
A'
6,508.06
A1/2
7,143.33
CI10
18,745
CImax
531
h10
65
A1
34.52
A3
10.11
Data for the last 5 years (citations for the last 10 years) on July 18, 2024; A3 for period 2018-2022
Data for ARIS tenders ( 04.04.2019 – Programme tender, archive )
Database Linked records Citations Pure citations Average pure citations
WoS  779  24,674  21,492  27.59 
Scopus  797  27,692  24,225  30.4 
Researchers (16)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  22616  PhD Tina Eleršek  Biology  Researcher  2020 - 2024  264 
2.  10582  PhD Ingrid Falnoga  Neurobiology  Researcher  2020 - 2024  348 
3.  12315  PhD Ester Heath  Control and care of the environment  Researcher  2020 - 2024  613 
4.  05027  PhD Milena Horvat  Chemistry  Researcher  2020 - 2024  1,900 
5.  33031  PhD Marina Klemenčič  Biochemistry and molecular biology  Researcher  2020 - 2024  138 
6.  27733  PhD Tina Kosjek  Control and care of the environment  Researcher  2020 - 2024  363 
7.  36323  PhD Tjaša Lukan  Biology  Researcher  2020 - 2021  127 
8.  18287  PhD Darja Mazej  Chemistry  Researcher  2020 - 2024  382 
9.  30063  PhD Janja Snoj Tratnik  Control and care of the environment  Researcher  2020 - 2024  296 
10.  37818  PhD Anja Stajnko  Biochemistry and molecular biology  Researcher  2020 - 2024  95 
11.  29635  Katja Stare  Biology  Technical associate  2020 - 2024  88 
12.  01411  PhD Zdenka Šlejkovec  Chemistry  Researcher  2020 - 2024  240 
13.  50566  PhD Špela Tomaž  Biotechnology  Researcher  2022 - 2023  28 
14.  39320  PhD Maja Zagorščak  Interdisciplinary research  Researcher  2020 - 2024  62 
15.  03950  PhD Dušan Žigon  Chemistry  Researcher  2020 - 2024  169 
16.  27522  PhD Anže Županič  Systems and cybernetics  Head  2020 - 2024  184 
Organisations (3)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,468 
2.  0103  University of Ljubljana, Faculty of Chemistry and Chemical Technology  Ljubljana  1626990  23,454 
3.  0106  Jožef Stefan Institute  Ljubljana  5051606000  91,936 
Abstract
For thousands of chemicals that are found in our environment little or no toxicological data are available. Current regulations, which are focused on physiological endpoints, are not suitable for the number of chemicals that require testing; the consensus of the (eco)toxicological research community is that a shift towards a more molecular mechanism-based, high-throughput toxicological testing is needed. The goal of the proposed research is to develop a method for assessing toxicological properties of chemicals, based on functional genomic screening of a pooled loss-of-function mutant library of the green alga Chlamydomonas reinhardtii and next-generation sequencing. C. reinhardtii was chosen as the model species based on the availability of the mutant library, existence of a well annotated genomic sequence, relative ease of cultivation and rapid growth, which will enable simultaneous screening of a large number of chemicals. The functional genomic profiles obtained after chemical exposure will serve as basis to (i) identify genetic determinants of tolerance and sensitivity to environmental chemicals, (ii) find and annotate as yet uncharacterized genes involved in chemical toxicity and (iii) classify the chemicals according to their mode of action. By screening a large number of chemicals of different chemical classes and combining the newly obtained and existing knowledge, a comprehensive set of chemical toxicity pathways and several adverse outcome pathways for C. reinhardtii will be developed, and shared with the research community in public databases (eg http://aopkb.org/). The project will draw from recent advances in molecular biology, sequencing and bioinformatics to develop high-quality resources and tools that will enable prioritization of chemical testing and accelerate the development of new mechanism based toxicological assays, thus contributing to a more objective and efficient assessment of chemical risk to the environment.
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