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Projects / Programmes source: ARIS

Biokemija membransko aktivnih snovi (Slovene)

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Periods
January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   
1.02.00  Natural sciences and mathematics  Physics   

Code Science Field
P004  Natural sciences and mathematics  Biochemistry, Metabolism 
P310  Natural sciences and mathematics  Proteins, enzymology 
P340  Natural sciences and mathematics  Lipids, steroids, membranes 
Evaluation (rules)
source: COBISS
Evaluation of bibliographic research performance indicators according to ARIS methodology
Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases
source: WoS source: Scopus source: COBISS
Researchers (9)
no. Code Name and surname Research area Role Period No. of publications
1.  15686  PhD Gregor Anderluh  Biochemistry and molecular biology  Researcher  2002 - 2003  968 
2.  21407  PhD Sabina Berne  Biotechnology  Researcher  2002 - 2003  165 
3.  23477  PhD Mojca Beseničar  Biochemistry and molecular biology  Researcher  2003  40 
4.  22470  PhD Katarina Črnigoj Kristan  Biochemistry and molecular biology  Researcher  2002 - 2003  35 
5.  06994  PhD Peter Maček  Biochemistry and molecular biology  Head  2002 - 2003  523 
6.  18203  PhD Petra Malovrh  Cardiovascular system  Researcher  1999 - 2002  43 
7.  17422  Irena Pavešič    Researcher  2002 - 2003  1 
8.  15328  PhD Kristina Sepčić  Biochemistry and molecular biology  Researcher  2002 - 2003  729 
9.  06905  PhD Tom Turk  Biochemistry and molecular biology  Researcher  2002 - 2003  619 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,295 
Abstract
The research program addresses naturally occurring toxic compounds that interfere with structure and function of biological membranes. Compounds are screened for those affecting integrity of lipid bilayers or may modify function of membrane proteins. The detected toxins are purified by using preparative techniques. Substances are biochemically characterized. Polypeptides are sequenced or their cDNA are prepared to analyze their primary structure. Another organic compounds are analyzed collaboratively for chemical properties and structure. Isolated compounds of interest, primarily polypeptides, are studied for their mechanism of toxic action on erythrocytes, other cells or artificial lipid vesicles if the substance expresses membrane damaging action. Basic science-oriented studies are aimed at highlighting of mechanisms of interactions of polypeptides with lipid and natural membranes. That knowledge may stimulate certain applied studies and development of immuno- and mitotoxins to be used in cancer and parasite treatment. It may also contribute to biological safety and human or animal health care. Utmost interest is oriented to peptides and proteins or other substances compromizing lipid or cell membranes. For example, representative are sea anemone cytolytic toxins and sea sponge 3-alkyl-pyridinium salts. Mechanism of membrane permeabilization is studied biochemically for toxin acceptors or receptors. Ligand affinity and kinetics of binding is determined. Using combined biochemical and biophysical methods, nature of membrane damages (for example, pores) is estimated. Conformational changes of toxic polypeptides are determined by using spectroscopy (fluorescence, circular dichroism). Using techniques of protein engineering proteins are modified by site-directed mutagenesis (point mutations, deletions) in order to study structure-function relationships.
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