Projects / Programmes source: ARIS

Mikrobna fiziologija in mikrobna ekologija (Slovene)

January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
4.03.00  Biotechnical sciences  Plant production   
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   
1.03.00  Natural sciences and mathematics  Biology   

Code Science Field
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
P004  Natural sciences and mathematics  Biochemistry, Metabolism 
B410  Biomedical sciences  Soil science, agricultural hydrology 
B433  Biomedical sciences  Fixation of nitrogen 
B260  Biomedical sciences  Hydrobiology, marine biology, aquatic ecology, limnology 
Evaluation (rules)
source: COBISS
Researchers (12)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  03156  MSc Polonca Čadež  Biochemistry and molecular biology  Researcher  2002 - 2003  64 
2.  22358  PhD Kaja Gnezda Meijer  Plant production  Researcher  2003 
3.  03383  PhD Ivan - Janez Hacin  Plant production  Researcher  2002 - 2003  112 
4.  22492  PhD Barbara Kraigher  Biology  Researcher  2003  111 
5.  17794  Simona Leskovec    Researcher  2002 - 2003  25 
6.  07043  PhD Ivan Mahne  Plant production  Head  2002 - 2003  200 
7.  05993  PhD Ines Mandić-Mulec  Biotechnology  Researcher  2002 - 2003  603 
8.  23543  PhD Duško Odić  Plant production  Researcher  2003  33 
9.  18148  PhD Marjan Peršuh  Biochemistry and molecular biology  Researcher  2002 - 2003  15 
10.  12466  PhD Alenka Prinčič  Animal production  Researcher  2002 - 2003  26 
11.  13005  PhD David Stopar  Plant production  Researcher  2002 - 2003  456 
12.  19104  PhD Blaž Stres  Animal production  Researcher  2002 - 2003  360 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  65,900 
Research activities of our group are focused on the regulation of the microbial processes involved in nitrogen metabolism on the physiological and ecological level. Mineralization/immobilization, nitrification, denitrification and molecular nitrogen fixation are studied in laboratory model systems and the results verified in complex natural environments such as soil and water. 1. Mineralization/immobilization: Environmental factors (pH, temperature, substrate, oxygen and water availability), controlling the conversion of mineral nitrogen into microbial biomas and its persistence in soil are of prime interest. 2. Nitrification: Preliminary results indicate that pH and oxygen availability affect the accumulation of nitrite during nitrification. Regulatory role of these two factors in nitritation and nitratation will be studied in detail. Accumulation of nitrite during nitrification is of biotechnological (practical) significance for the removal of excess nitrogen in waste waters by simultaneous nitrification and denitrification 3. Denitrification: Dissimilatory nitrate reduction is enabled by membrane bound nitrate reductase (NR). In addition to NR, some bacterial strains posses periplasmic nitrate reductase (NAP) the physiological role of which is as yet not entirely understood. Our research group has isolated NAP from Pseudomonas stutzeri. Physiological role of the enzyme will be studied in the future, using nap mutants. 4. Biological nitrogen fixation: Studies are concerned with: a) criteria for the selection of superior symbiotic partners in R.leguminosarum/Phaseoulus and R. meliloti/Trigonella symbioses, b) liquid inoculant formulations of Rhizobium/Bradyrhizobium spp., and c) the role of fixed nitrogen in nitrogen balance of soil and nonsymbiotic plants. 5. Microbial communities: The structure of microbial community participating in nitrogen transformations in soil and water is being studied in relation to environmental factors, using molecular biology techniques. The effect of viruses on bacterial population and thereby on the flow of nitrogen, carbon and other nutrients are being examined. The effect of host energy status on prophage induction is studied on the molecular level. The role of horizontal and vertical gene transfer in the structure and dynamics of microbial communities will be investigated. The research is focused on pheromone signaling and regulation of development of genetic competence in selected bacterial strains isolated from the environment.
Significance for science
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
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