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Projects / Programmes source: ARIS

Uravnavanje genske ekspresije s stereoidi in uporaba genske tehnologije P450 v medicini (Slovene)

Periods
January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   

Code Science Field
P004  Natural sciences and mathematics  Biochemistry, Metabolism 
P320  Natural sciences and mathematics  Nucleic acids, protein synthesis 
Evaluation (rules)
source: COBISS
Researchers (12)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  03079  PhD Katja Breskvar  Biochemistry and molecular biology  Head  2001 - 2003  189 
2.  19879  PhD Boštjan Černila  Biochemistry and molecular biology  Researcher  2001 - 2003  23 
3.  08777  PhD Bronislava Črešnar  Biochemistry and molecular biology  Researcher  2001 - 2003  98 
4.  11711  PhD Vita Dolžan  Biochemistry and molecular biology  Researcher  2001 - 2003  768 
5.  22458  PhD Darja Herman  Biochemistry and molecular biology  Researcher  2002 - 2003  23 
6.  21494  PhD Nataša Jeraj Kampuš  Biochemistry and molecular biology  Researcher  2001 - 2003  20 
7.  15848  PhD Barbara Kunič  Biochemistry and molecular biology  Researcher  2001 - 2002  20 
8.  18847  Marjan Kužnik    Researcher  2001 - 2003 
9.  02934  PhD Helena Lenasi  Biochemistry and molecular biology  Researcher  2001 - 2003  78 
10.  07085  PhD Tomaž Makovec  Biochemistry and molecular biology  Researcher  2001 - 2003  48 
11.  07086  Savica Soldat    Researcher  2001 - 2003 
12.  19132  PhD Tanja Vilfan  Biochemistry and molecular biology  Researcher  2001 - 2003  14 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,701 
Abstract
a. The mechanism of induction of steroid inducible enzyme systems in filamentous fungus R.nigricans was studied on DNA level and on the level of membrane receptors. It was confirmed that induction process could involve membrane bound G-proteins and phosphorylation of cellular proteins. The presence of progesterone receptors and G-proteins was demonstrated in the plasma membrane fraction . The GTPase activity of G-proteins increased after treatment with progesterone. The involvement of protein kinases was studied indirectly using genistein the inhibitor of protein kinases. Fungal cells tested for the induction ability in the presence of kinase inhibitor exhibited significantly lower hydroxylation activity ( 43%) when compared to the control samples. Phosphorylation of cellular proteins was studied also directly using radioactively labelled ATP. Phosphorylated proteins were analysed by PAGE method and it was shown that the detection of radioactively labelled phosphorylated proteins was possible but the additional methodological improvements are needed. Concerning the studies on DNA level we isolated and characterised two different cDNA clones containing information for cytocolic stress proteins from Hsp70 family. It was also shown on RNA level that expression of the above stress proteins increased by the action of testosterone and deoxycorticosterone. The expression was also influenced by temperature stress and oxidative stress. b. The detoxification and activation of many xenobiotics in man are catalysed by polymorphic cytochrome P450 enzymes. The polymorphism of corresponding genes was studied in correlation with the genes responsible for activating as well as detoxifying enzymes. Interindividual variability due to genetic polymorphism was used to evaluate susceptibility to human cancer caused by chemical carcinogenesis.
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Most important socioeconomically and culturally relevant results Final report
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