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Projects / Programmes source: ARIS

Prion diseases and their diagnostics

Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
B500  Biomedical sciences  Immunology, serology, transplantation 
B640  Biomedical sciences  Neurology, neuropsychology, neurophysiology 
B750  Biomedical sciences  Veterinary medicine: surgery, physiology, pathology, clinical studies 
B210  Biomedical sciences  Histology, cytochemistry, histochemistry, tissue culture 
B490  Biomedical sciences  Haematology, extracellular fluids 
B120  Biomedical sciences  Molecular biophysics 
P310  Natural sciences and mathematics  Proteins, enzymology 
T490  Technological sciences  Biotechnology 
Keywords
monoclonal antibodies, prions, prion diseases, diagnostics, genotyping, NMR
Evaluation (rules)
source: COBISS
Researchers (21)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  18675  Robert Bremšak    Researcher  2002 - 2004  11 
2.  10428  PhD Mara Bresjanac  Neurobiology  Researcher  2002 - 2004  251 
3.  05236  PhD Vladka Čurin Šerbec  Microbiology and immunology  Head  2002 - 2004  264 
4.  12449  PhD Robert Frangež  Veterinarian medicine  Researcher  2002 - 2004  284 
5.  15455  PhD Vesna Galvani  Microbiology and immunology  Researcher  2002 - 2004  69 
6.  09275  PhD Damjan Glavač  Chemistry  Researcher  2002 - 2004  565 
7.  15978  PhD Katrina Hartman  Microbiology and immunology  Researcher  2002 - 2004  45 
8.  06628  PhD Roman Jerala  Biochemistry and molecular biology  Researcher  2002 - 2004  1,202 
9.  04630  PhD Polona Juntes  Veterinarian medicine  Researcher  2002 - 2004  426 
10.  17917  PhD Andreja Majerle  Biotechnology  Researcher  2002 - 2004  92 
11.  01644  PhD Milan Pogačnik  Veterinarian medicine  Researcher  2002 - 2004  624 
12.  07090  PhD Mara Popović  Neurobiology  Researcher  2002 - 2004  306 
13.  16340  PhD Uroš Potočnik  Microbiology and immunology  Researcher  2002 - 2004  634 
14.  12060  PhD Primož Pristovšek  Chemistry  Researcher  2002 - 2004  135 
15.  15103  MSc Ruth Rupreht  Microbiology and immunology  Researcher  2002 - 2004  90 
16.  12266  PhD Marjan Slak Rupnik  Metabolic and hormonal disorders  Researcher  2002 - 2004  350 
17.  18884  PhD Tanja Švara  Veterinarian medicine  Researcher  2002 - 2004  229 
18.  21335  PhD Tanja Vranac  Biochemistry and molecular biology  Researcher  2002 - 2004  40 
19.  13334  PhD Milka Vrecl Fazarinc  Veterinarian medicine  Researcher  2002 - 2004  272 
20.  08506  PhD Jelka Zabavnik Piano  Veterinarian medicine  Researcher  2002 - 2004  204 
21.  04651  MSc Teofil Žižek  Chemistry  Researcher  2002 - 2004  14 
Organisations (4)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  21,237 
2.  0311  Blood Transfusion Centre of Slovenia  Ljubljana  5053960  1,759 
3.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,704 
4.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  10,934 
Abstract
Prioni diseases represent a threat to public health, while their effect on European economy amounts to billions of dollars. Due to the long incubation period and the unknown mode of the interspecies transfer and advancement of the disease it is impossible to asses accurately the extent of threatening epidemics in humans. Reliable diagnostics is therefore of extreme importance to prevent spreading of prioni diseases. All currently available diagnostic tests are based on antibodies which do not distinguish between normal (PrPc) and pathological form of prion protein (PrPSc). Ideal test should be able to distinguish between the two forms, which would make it simple, fast and also less expensive. Based on monoclonal antibodies (MAbs), prepared at the Blood Transfusion Centre of Slovenia against a peptide fragment chosen from the primary structure of a human PrP, we will develop and validate a reliable test for diagnostics of prion diseases within the scope of the proposed project. Furthermore, we will use different MAbs for research of human and animal prion proteins in various tissues and body fluids, in the first place in human blood. We will determine the frequency of the codon 129 polymorphism of PrP gene in Slovenian population and try to evaluate its role in the prion disease. MAbs will be also used for research of molecular mechanisms of recognition of different forms of PrP. Based on NMR experiments on isotopically labelled peptide, we will monitor its conformation in complex with different antibody fragments, which recognize either native or pathologically modified protein. This will allow us to infer the conformation of pathologically modified protein or the conformational change, which causes the aggregation of prion protein on the onset of the disease. We will also investigate the response of prion protein to changes in solution conditions, such as pH, polarity of the solvent, ionic strength etc..
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