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Projects / Programmes source: ARIS

Application of high throughput techniques for analysis of gene expression in plant-pathogen and plant-herbivore interactions

Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
Keywords
potato, Colorado potato beetle, viral pathogens, plant defence mechanism, gene expression, biological control, DNA microarrays
Evaluation (rules)
source: COBISS
Researchers (8)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  19994  MSc Tina Demšar  Biotechnology  Researcher  2002 - 2004  187 
2.  12688  PhD Kristina Gruden  Biotechnology  Head  2002 - 2004  981 
3.  04333  PhD Maja Kovač  Biotechnology  Researcher  2002 - 2004  204 
4.  18467  PhD Maruša Pompe Novak  Biotechnology  Researcher  2002 - 2004  289 
5.  05229  PhD Maja Ravnikar  Biotechnology  Researcher  2002 - 2004  1,355 
6.  21551  PhD Nina Slapar  Pharmacy  Researcher  2002 - 2004  21 
7.  07849  PhD Borut Štrukelj  Biochemistry and molecular biology  Researcher  2002 - 2004  1,120 
8.  05234  Mojca Trstenjak Prebanda  Biochemistry and molecular biology  Researcher  2002 - 2004  64 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,295 
2.  0106  Jožef Stefan Institute  Ljubljana  5051606000  89,961 
Abstract
Plant pathogens and pests are a cause of substantial yield loss throughout the world. For example, damage caused by pests is estimated to be app. 100 billions USD per year despite intensive use of pesticides. Understanding the principles of plant-pathogen and plant-herbivore interactions is a possible approach to counter this problem. Up to now, it has been possible to follow the interactions on a level of only one or a few genes at a time. Analysis of gene expression by DNA microarrays allows us to follow over thousand genes at a time, making it possible to produce a more comprehensive picture of physiological processes and, through it, enabling us to find potential targets for plant protection in a more directed manner.We will analyse the response of potato cultivars with different susceptibility to infection by virus PVYNTN. A library of differentially expressed genes in infected and noninfected potato leaves will be constructed. The expression of 400 randomly picked genes from that library will be analysed by DNA microarrays. Their expression after local, systemic infection and established secondary infection will be determined. The process of Colorado potato beetle larvae adaptation to ingestion of plant defence molecules will be studied using the same approach. Time scale of this process occurring in the guts of larvae will be followed. Additionally, tritrophic interaction of all three organisms will be investigated under conditions simulating the field situation. Selected genes from potato and Colorado potato beetle larvae will be isolated and their potential in plant protection will be evaluated by bioassays. Once established, this advanced method can be readily adapted to analysis of other agriculturally or industrially important organisms, resulting in novel, effective and environmentally friendly tools for plant disease management.
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