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Projects / Programmes source: ARIS

Molecular Mechanisms in Muscle Development and Function

Periods
Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
B007  Biomedical sciences  Medicine (human and vertebrates) 
B640  Biomedical sciences  Neurology, neuropsychology, neurophysiology 
Keywords
skeletal muscle, mammals, plasticity, regeneration, ageing, myosin heavy chains, capillaries, satellite cells, myonuclei, neuromuscular junction, acetilcholinesterase mRNA, agrin,culture of human skeletal muscle, insulin resistence, RT-PCR, in situ hybridisation, muscle diseases
Evaluation (rules)
source: COBISS
Researchers (25)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  17930  Ivan Blažinovič    Technical associate  2004 - 2008 
2.  11903  PhD Martina Brank  Biotechnology  Researcher  2004 - 2008  28 
3.  17908  PhD Janez Brecelj  Neurobiology  Researcher  2004 - 2008  116 
4.  10644  PhD Erika Cvetko  Neurobiology  Researcher  2004 - 2008  278 
5.  11785  PhD Vita Čebašek  Neurobiology  Researcher  2005 - 2008  66 
6.  21610  Majda Črnak-Maasarani    Technical associate  2004 - 2008 
7.  05329  PhD Ida Eržen  Neurobiology  Head  2004 - 2008  212 
8.  07089  Zvonka Frelih    Technical associate  2004 - 2008 
9.  19129  PhD Marko Goličnik  Neurobiology  Researcher  2004 - 2008  143 
10.  04410  PhD Zoran Grubič  Neurobiology  Researcher  2004 - 2008  384 
11.  22403  PhD Marko Jevšek  Neurobiology  Researcher  2004 - 2008  117 
12.  16345  PhD Tomaž Marš  Neurobiology  Researcher  2004 - 2008  343 
13.  04905  PhD Marija Meznarič  Neurobiology  Researcher  2004 - 2008  151 
14.  19318  PhD Katarina Miš  Neurobiology  Researcher  2004 - 2008  191 
15.  27571  PhD Robert Pal  Neurobiology  Junior researcher  2006 
16.  29470  PhD Katarina Pegan  Biochemistry and molecular biology  Junior researcher  2008  44 
17.  28351  PhD Sergej Pirkmajer  Neurobiology  Junior researcher  2007 - 2008  442 
18.  21806  PhD Matej Podbregar  Cardiovascular system  Researcher  2004 - 2008  330 
19.  26057  Nataša Pollak    Technical associate  2005 - 2008 
20.  24281  PhD Oja Prelovšek  Neurobiology  Junior researcher  2005 - 2008  16 
21.  28168  Polona Sajovic    Technical associate  2007 - 2008 
22.  17961  Marko Slak    Technical associate  2004 - 2008 
23.  10641  PhD Viktorija Smerdu  Neurobiology  Researcher  2004 - 2008  90 
24.  19215  Friderik Štendler    Technical associate  2004 - 2008 
25.  17963  Anica Tomažinčič    Technical associate  2004 - 2005 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0312  University Medical Centre Ljubljana  Ljubljana  5057272000  75,513 
2.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  45,384 
Abstract
The program joins two research groups: 'Skeletal muscle: its structure and function'and 'Human muscle under the in vitro conditions'. The program proposed by group A is dealing with the morphological, morphometric and immunohistochemical analysis of normal and diseased muscles. It consists of three research directions: I. Morphological and histochemical characteristics of skeletal muscles: We shall continue investigating the morphology and the expression pattern of myosin heavy chain isoforms (MyHC) and their transcripts in skeletal muscles of bigger mammals. Human extraocular muscles will be analysed in different age periods to prove that human EOM do not age. Special emphasis will be given to the capillary density per muscle fibre. II. Molecular mechanisms underlying the acute myopathic changes, accompanying critical conditions. Recent investigations revealed active role of the skeletal muscle fibre in the signalling processes, triggered by different types of cytokines under the stress conditions like septic shock. In our preliminary studies we demonstrated release of IL-6 from the cultured human skeletal muscle fibre after the treatments with TNF-a. These investigation will be continued in this part of the program. III. Molecular mechanisms responsible for the insulin resistance in the human skeletal muscle fibre. Insulin resistance is the major cause of hyperglycemia in type 2 diabetics and is therefore intensively investigated. The experimental model of the in vitro innervated human muscle allows studies of the molecular mechanisms underlying insulin-induced exocytosis, which is impaired in the insulin-resistant muscle fibres. In collaboration with the research group at the Institute of Pathophysiology, which is fully equipped to follow the processes of exocytosis, we shall investigate molecular mechanisms underlying the insulin-induced translocation of the glucose transporters 4 (GLUT4) bearing vesicles to the II. Plasticity of skeletal muscle in physiological, experimental and pathological conditions Plasticity of skeletal muscles that has been thoroughly studied in our previous research will further be investigated either on experimental models, like heterochronous isotransplantation, heterotopic transplantation of the rat slow and fast muscles as well as axotonotomy and axonotmesis. Additionally, plasticity will be studied in human muscles in inactivity and in the critical illness myopathy. Special attention will be paid to the influence of the changed innervation, chorticosteroid and thyroid hormones on the expression of the myosin heavy chain isoforms and their transcripts as well as to the myogenic factors and to the changed capillary supply. A part of our research will be devoted to the investigation of the biophysical characteristics of normal and dystrophic muscles and to the morphometric analysis of the muscles in patients with the myotonic dystrophy III Epidemiology of the limb-girdle muscular dystrophy We shall continue to study the epidemiology of the 'limb-girdle dystrophy' in Slovenia. The research program proposed by group B is focused on the molecular mechanisms underlying various aspects of the development and function of the human skeletal muscle fibre. In most studies we will employ the in vitro system in which human muscle fibre is innervated by the motor neuron extending from the explant of the embryonic rat spinal cord. The main research directions will be the following: I. Molecular mechanism regulating synaptogenesis and function of the neuromuscular junction (NMJ) By "gene silencing" we will investigate the roles and expression of specific proteins participating in the formation and maintenance of the NMJ. This part of our research will be focused on agrin, AChE and BuChE, which are all important, basal lamina bound constituents of the NMJ. This part of our program includes continuation of our previous research on the structure - function relationship of the AC...
Significance for science
Understanding at the molecular level the mechanisms underlying functioning of the human organism in normal and pathologic conditions is one of the major research challenges in modern medicine. This orientation has provided the rational basis for approaches and techniques that have significantly improved diagnosis and treatment of various diseases. As evident from our publications we folloved this orientation and conributed new knowledge on the morphology, functioning, development, differentiation and plasticity of the skeletal muscle in the normal and selected diseased states
Significance for the country
Our research contributed to the development of Slovenia in the following ways: - it contributed to the recognition of Slovenia at the international level.in the field of neurobiological research This has been achieved by establishing contacts with the top laboratories in the world and by publishing our results in the peer reviewed international journals (see bibliography of the researchers of the group). - by participating in teaching and training of students at the highest levels of Slovenian education system: researches of the group participated in courses for graduate, postgraduate and Ph. D. students of medicine, pharmacy and other biological fields. Many of them were directly included in our research . - by introducing and developing new approaches and techniques; some of them were applied in the clinical practice in the diagnostics of neuromuscular diseases.
Most important scientific results Final report, complete report on dLib.si
Most important socioeconomically and culturally relevant results Final report, complete report on dLib.si
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