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Projects / Programmes source: ARIS

Chemical and biological monitoring of neonicotinoids and their impact assessment in the environment

Research activity

Code Science Field Subfield
1.08.00  Natural sciences and mathematics  Control and care of the environment   

Code Science Field
P305  Natural sciences and mathematics  Environmental chemistry 
B361  Biomedical sciences  Physiology of invertebrates 
Keywords
neonicotinoids, imidacloprid, persistence, metabolites,animals,toxicity
Evaluation (rules)
source: COBISS
Researchers (12)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  26249  Aleš Boškin  Control and care of the environment  Technical associate  2006 - 2007  16 
2.  14136  MSc Maja Černilec  Microbiology and immunology  Researcher  2006 - 2007  51 
3.  11155  PhD Damjana Drobne  Biology  Researcher  2005 - 2007  863 
4.  04537  PhD Mladen Franko  Control and care of the environment  Researcher  2005 - 2007  608 
5.  15455  PhD Vesna Galvani  Microbiology and immunology  Researcher  2005 - 2007  69 
6.  24447  PhD Anita Jemec Kokalj  Biology  Junior researcher  2005 - 2007  291 
7.  11873  PhD Urška Lavrenčič Štangar  Chemistry  Researcher  2005 - 2007  559 
8.  17263  Emil Meden    Technical associate  2005 - 2007  109 
9.  03588  PhD Tatjana Tišler  Control and care of the environment  Researcher  2005 - 2007  370 
10.  11539  PhD Polonca Trebše  Control and care of the environment  Head  2005 - 2007  514 
11.  21335  PhD Tanja Vranac  Biochemistry and molecular biology  Researcher  2006 - 2007  40 
12.  17107  PhD Irma Vuk  Biotechnical sciences  Researcher  2005 - 2007  90 
Organisations (4)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  20,958 
2.  0311  Blood Transfusion Centre of Slovenia  Ljubljana  5053960  1,737 
3.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,295 
4.  1540  University of Nova Gorica  Nova Gorica  5920884000  14,063 
Abstract
The aim of our research is to study the degradation of neonicotinoids in the environment. As a part of the research we will investigate photochemical degradation in water (reactions caused by natural sunlight, reactions using mercury lamp). As the model compound we will use imidacloprid. We will also trace the degradation processes of imidacloprid in soil and in vegetables under natural conditions. Beside this we will try to determine the degradation products and metabolites and try to establish, how they incorporate in the environment. On this basis we will try to propose the mechanism of degradation. For the isolation and detection we will use classical analytical methods, such as solid phase extraction and liquid-liquid or solid-liquid extraction, in combination with gas and liquid chromatography with different detectors (MS, DAD).Results of the degradation of imidacloprid will be combined with toxicity tests for terrestrial isopods and with standard toxicity test with D magna. During two or four weeks of experiments with terrestrial isopods we will follow weight, assimilation efficiency and mortality. At the end of experiments some of biochemical parameters, such as acetylcholinesterase activity, glutathion-s-transferase activity, lipids, proteins and glycogen wiil be determined after the exposure to imidacloprid in food. Acute toxicity of imidacloprid will be determined with establishing of normal movement of D. Magna. In chronic test we will follow their reproduction. In this research we want to make a comparison in behaviour of organophosphates and neonicotinoids in the environment. This can be done by using already existiong methods for the determination of concentrations and on the basis of toxicity tests for carbamates and organophosphates, developed also in our laboratory. We want to find out, if the replacement of organophosphates with neonicotinoids in suitable and what kind of concequences it might have for nontarget organisms and lifeless nature and also for the mankind.
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