Projects / Programmes
The use of cultured skin substitutes for healing chronic and acute wounds
| Code |
Science |
Field |
Subfield |
| 7.00.00 |
Interdisciplinary research |
|
|
| Code |
Science |
Field |
| B740 |
Biomedical sciences |
Pharmacological sciences, pharmacognosy, pharmacy, toxicology |
| B600 |
Biomedical sciences |
Surgery, orthopaedics, traumatology |
skin substitute, fibrin matrix, allogeneic and autologous skin cells, chronic and acute wounds
Researchers (14)
Organisations (3)
Abstract
Innapropriate healing of chronic and acute post-traumatic wounds represents a major problem mainly for elderly patients and may culminate in an amputation of the affected part of a limb. The outstanding progress in the field of tissue engineering and cell therapies has opened numerous possibilities and one of them is the use of cultured allogeneic or autologous skin substitutes in the treatment of such peristant skin lesions. We will use optimized fibrin-based skin substitutes, prepared with our own technology in such a way that they contain only small amounts of animal origin proteins, to treat patients with chronic and acute wounds.We expect that by applying this new therapy we will be very successful in most cases and that through our research we will be able to select the most approprate type of a skin substitute (autologous, allogeneic) to be used for the stated purpose. Additionally we will test differential effects of various commercially available fibrin glues, selected collagen matrix and the addition of thrombocyte concetrate on keratinocyte proliferation in vitro as well as their suitability for the production of clinically applicable skin substitutes. The technology of epidermal and combined epidermal-dermal skin substitute production enables us to prepare organotypic skin models to be used as test systems for evluating negative or positive effects of various topically applied substances. Such effects can be monitored in different ways, for example microscopically or by using molecular biology techniques. By applying organotypic skin models we won't need experimental animals and will be able to study the mechanisms of action of a given xenobiotic, in our case calcipotriol, the analogue of vitamin D3.
