Projects / Programmes source: ARIS

Ecomical exploitation of slaughtering house blood

Research activity

Code Science Field Subfield
4.06.03  Biotechnical sciences  Biotechnology  Animal biotechnology 
Polymeric membranes, surface modification; blood separation, cells, proteines; citokines.
Evaluation (rules)
source: COBISS
Researchers (8)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  14488  PhD Avrelija Cencič  Biotechnology  Researcher  1998 - 1999  458 
2.  05320  PhD Bratko Filipič  Biochemistry and molecular biology  Researcher  1998 - 1999  262 
3.  16128  Vesna Lahovnik  Materials science and technology  Researcher  1998 - 1999  24 
4.  16127  Leonida Oberš  Mathematics  Researcher  1998 - 1999 
5.  09467  PhD Vladimir Strelec  Animal production  Researcher  1998 - 1999  186 
6.  03033  PhD Črtomir Stropnik  Chemistry  Head  1998 - 1999  334 
7.  09472  PhD Marko Volk  Veterinarian medicine  Researcher  1998 - 1999  462 
8.  16112  Evgen Zgubič  Animal production  Researcher  1998 - 1999  35 
Organisations (3)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  45,084 
2.  0482  University of Maribor, Faculty of Agriculture and Life Sciences  Hoče  5089638004  9,556 
3.  0794  University of Maribor, Faculty of Chemistry and Chemical Engineering  Maribor  5089638012  12,560 
On the basis of our knowledge on polymeric asymmetric porous membrane formation by wet phase separation we prepared membranes for separation of blood cells from the blood and further separation of proteines. With the wet phase separation we prepared several microfiltration membranes from different polymeric materials. With addition to improve separation characteristics we modified the surface of some polymeric membranes. Apart from the water (82%), blood contains blood cells (around 10%), proteins (around 7%) and electrolites, sugars and so on (less then 1 %). Blood ingridents are named by their size: the order of magnitude of cells size varies from two to over ten micrometers, the order of magnitude of protein macromolecules size is some ten nanometers and the order of magnitude of small molecules and ions do notexceed the value of some nanometers. On the same basis the membrane separation processes are clasificated as microfiltration, ultrafiltration and nanofiltratin or reverse osmosis. With laboratory prepared membranes we separated porcine blood by dead end filtration. With several membranes we were able to separate blood cells from plasma. In the permeate we determined the concentration of proteines. There will be done more in the field of protein separation and determination with SDS electrophoresis to determine which proteines pass through the membrane and which are retained at the membrane surface. In the future we plan to use the cross flow filtration to minimize the concentration polarization and fouling.
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