Projects / Programmes
Ecomical exploitation of slaughtering house blood
Code |
Science |
Field |
Subfield |
4.06.03 |
Biotechnical sciences |
Biotechnology |
Animal biotechnology |
Polymeric membranes, surface modification; blood separation, cells, proteines; citokines.
Researchers (8)
no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
1. |
14488 |
PhD Avrelija Cencič |
Biotechnology |
Researcher |
1998 - 1999 |
458 |
2. |
05320 |
PhD Bratko Filipič |
Biochemistry and molecular biology |
Researcher |
1998 - 1999 |
262 |
3. |
16128 |
Vesna Lahovnik |
Materials science and technology |
Researcher |
1998 - 1999 |
24 |
4. |
16127 |
Leonida Oberš |
Mathematics |
Researcher |
1998 - 1999 |
8 |
5. |
09467 |
PhD Vladimir Strelec |
Animal production |
Researcher |
1998 - 1999 |
186 |
6. |
03033 |
PhD Črtomir Stropnik |
Chemistry |
Head |
1998 - 1999 |
334 |
7. |
09472 |
PhD Marko Volk |
Veterinarian medicine |
Researcher |
1998 - 1999 |
462 |
8. |
16112 |
Evgen Zgubič |
Animal production |
Researcher |
1998 - 1999 |
35 |
Organisations (3)
Abstract
On the basis of our knowledge on polymeric asymmetric porous membrane formation by wet phase separation we prepared membranes for separation of blood cells from the blood and further separation of proteines. With the wet phase separation we prepared several microfiltration membranes from different polymeric materials. With addition to improve separation characteristics we modified the surface of some polymeric membranes.
Apart from the water (82%), blood contains blood cells (around 10%), proteins (around 7%) and electrolites, sugars and so on (less then 1 %). Blood ingridents are named by their size: the order of magnitude of cells size varies from two to over ten micrometers, the order of magnitude of protein macromolecules size is some ten nanometers and the order of magnitude of small molecules and ions do notexceed the value of some nanometers. On the same basis the membrane separation processes are clasificated as microfiltration, ultrafiltration and nanofiltratin or reverse osmosis.
With laboratory prepared membranes we separated porcine blood by dead end filtration. With several membranes we were able to separate blood cells from plasma. In the permeate we determined the concentration of proteines. There will be done more in the field of protein separation and determination with SDS electrophoresis to determine which proteines pass through the membrane and which are retained at the membrane surface. In the future we plan to use the cross flow filtration to minimize the concentration polarization and fouling.