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Projects / Programmes source: ARRS

Preparation of fungal antigens and study of their immunogenic properties for diagnostic application

Research activity

Code Science Field Subfield
4.04.02  Biotechnical sciences  Veterinarian medicine  Animal pathology and epizootiology 

Code Science Field
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
P004  Natural sciences and mathematics  Biochemistry, Metabolism 
Keywords
dermatophytoses, M. canis, immunogenicity, antigens, diagnostic methods, zoonotic diseases
Evaluation (rules)
source: COBISS
Researchers (7)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  07674  PhD Darja Barlič Maganja  Medical sciences  Researcher  1998 - 1999  423 
2.  08220  PhD Vojislava Bole-Hribovšek  Biotechnical sciences  Researcher  1998 - 1999  124 
3.  07850  PhD Marinka Drobnič-Košorok  Biotechnical sciences  Principal Researcher  1998 - 1999  184 
4.  18122  Boštjan Drolc  Biotechnical sciences  Researcher  1998 - 1999  10 
5.  09654  PhD Silvestra Kobal  Biotechnical sciences  Researcher  1998 - 1999  260 
6.  11132  PhD Branko Krt  Biotechnical sciences  Researcher  1998 - 1999  226 
7.  00902  PhD Valentin Skubic  Biotechnical sciences  Researcher  1998 - 1999  129 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  9,902 
Abstract
Fungal skin infections are permanently increasing in the countries of southern Europe as well as in Slovenia and its neighbour countries. Among them the infection with Microsporum canis is the most widely spread. Standard diagnostic methods are time consuming and some times unreliable compared to culture techniques. The efforts of different research groups are focussed on study and understanding of specific immunogenic defence mechanisms associated with mycoses, including the mechanisms of antigen presentation, lymphocyte activation and mechanisms responsible for elimination of antigens. Studies of natural and experimental fungal infections have revealed that both, humoral and cellular immune response are activated during the infection. However, in the case of dermatomycoses cellular immune response is supposed to have a protective role. Cell wall proteins, metabolite as well as intracellular proteins represent the potential antigens for the invaded host organism. Cell wall proteins belong mainly to glycoproteins while among metabolites and intracellular proteins different proteinases, specially keratinases seem to be the most important during the spread of the infection. Isolation and characterisation of these protein components may enable the development of new serologic diagnostic tools as well as identification of the most immunogenic proteins which can be candidates for incorporation in a new protective vaccine. Permanent increase of fungal infections suggested us to study the role of particular fungal antigens in the cases of epidemiologically and epizootiologically most frequently observed infections with dermatophytes like Microsporum canis and Trichophyton mentagrophytes and yeast belonging to genus of Malassezia and Candida. to find the most immunogenic proteins, the mechanically disrupted micelia will be extracted in buffer solutions and further analysed by chromatographic and electrophoretic procedures. The specific antigens isolated will be used in studies of humoral and cellular immune response associated with different fungal infections and compared with commercially available allergens. These studies are supposed to reveal possible connections between patogenicity and antigenic protein profile of the fungi studied.
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