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Projects / Programmes source: ARRS

Improving immune response of animals against Mycobacterium avium subsp. paratuberculosis and Clostridium perfringens toxins with chicken egg yolk antibodies

Research activity

Code Science Field Subfield
4.04.00  Biotechnical sciences  Veterinarian medicine   

Code Science Field
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 

Code Science Field
4.03  Agricultural and Veterinary Sciences  Veterinary science 
Keywords
bacterial toxins, PFO, NetB, Clostridium perfringens, immunization, Yolk derived IgY, Mycobacterium avium subsp. paratuberculosis
Evaluation (rules)
source: COBISS
Researchers (21)
no. Code Name and surname Research area Role Period No. of publications
1.  28448  PhD Jana Avberšek  Veterinarian medicine  Researcher  2018 - 2021  106 
2.  20491  Marko Bogataj    Technician  2018 - 2021 
3.  18325  PhD Simon Caserman  Biochemistry and molecular biology  Researcher  2018 - 2021  105 
4.  14880  PhD Igor Gruntar  Veterinarian medicine  Researcher  2018 - 2021  107 
5.  39711  Maja Jamnik    Technician  2018 - 2021  19 
6.  39657  Maja Kavalič    Technician  2018 - 2021 
7.  38142  PhD Tanja Knific  Veterinarian medicine  Researcher  2019 - 2021  61 
8.  23320  PhD Uroš Krapež  Veterinarian medicine  Researcher  2018 - 2021  164 
9.  21703  Darja Krelj    Technician  2020 - 2021 
10.  24296  PhD Darja Kušar  Veterinarian medicine  Researcher  2018 - 2021  177 
11.  17276  Jelka Lenarčič    Technician  2018 - 2020 
12.  11133  PhD Matjaž Ocepek  Veterinarian medicine  Principal Researcher  2018 - 2021  460 
13.  24612  PhD Mateja Pate  Veterinarian medicine  Researcher  2018 - 2019  273 
14.  24598  PhD Tina Pirš  Veterinarian medicine  Technician  2018 - 2021  82 
15.  22446  PhD Brigita Slavec  Veterinarian medicine  Researcher  2018 - 2021  184 
16.  18884  PhD Tanja Švara  Veterinarian medicine  Researcher  2018 - 2021  211 
17.  29352  PhD Marko Zadravec  Veterinarian medicine  Researcher  2018 - 2020  181 
18.  33447  Urška Zajc  Veterinarian medicine  Technician  2018 - 2021  72 
19.  12682  PhD Irena Zdovc  Veterinarian medicine  Researcher  2018 - 2021  452 
20.  08023  PhD Olga Zorman Rojs  Veterinarian medicine  Researcher  2018 - 2021  403 
21.  16244  PhD Petra Zrimšek  Veterinarian medicine  Researcher  2018 - 2021  202 
Organisations (2)
no. Code Research organisation City Registration number No. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  20,033 
2.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  9,696 
Abstract
Oral administration of specific antibodies is one of the possible alternatives how to approach the gastrointestinal infections in humans and animals. Similarly as vaccination, it shows several benefits in comparison to the conventional antibiotic therapy: It does not lead to the development of the resistance of microbes to antimicrobial drugs and may also be used preventively; It can act both against bacterial and viral infections; It does not affect the normal gut microbiota; There are no drug residua in the treated animals. Compared with vaccination, the oral administration of specific antibodies is often a simpler and cheaper option. In their production there are no requirements related to the recombinant technology and no public resistance in relation to the genetically modified organisms. The production of egg proteins is technologically and cost-effective, and easily scalable, while it is non-invasive and therefore acceptable to the general public. Also, the application itself is, as a rule, simpler and cheaper. In addition, it does not induce a non-specific immune response that may interfere with the use of vaccines. In the scope of the project, we aim to develop the technology for the production and use of IgY for the control and prevention of two of the economically extremely important gastrointestinal diseases. Namely: paratuberculosis - incurable diseases of ruminants, and necrotic enteritis - one of the most common diseases of fattening poultry. Hens will be immunized with a pre-prepared inactivated antigen from the paratuberculosis agent and also with the inactivated toxins PFO and NetB. From the egg yolks, IgY will be isolated and their quantity and specificity verified. They will be encapsulated and administered per os to the experimental animals, which will be exposed to the agents causing paratuberculosis and necrotic enteritis to assess the effectiveness of the acquired protection. Thus, as the first, we would develop a technology for the use of IgY antibodies to protect animals from Map. An effective method for the prevention or control of paratuberculosis would be a significant breakthrough in the fight against one of the most economically important ruminant diseases worldwide. The production of such an effector also represents a great economic opportunity. Also, the preparation and effective use of IgY antibodies against PFO and NetB toxins will be a completely new and original contribution to the science, as this has not yet been done according to the available data in the literature. In addition, for the disease prevention, the development of this non-invasive method, which practically has not been used yet currently for the disease control in the EU, is an extremely important contribution to the development of the field of profession. Namely, it can be expected in the near future that the importance of using alternatives to antibiotic therapy will greatly increase due to the increased resistance of bacteria to antimicrobial drugs. Thus, the experiences that will be gained during the proposed project will also be important in the development and implementation of this technology for the control of other diseases.
Significance for science
The production and use of antibodies derived from chicken eggs has been gaining in importance in the recent years. Despite the fact that procedures for the prevention or treatment of various bacterial and viral gastrointestinal diseases of domestic animals have already been developed, there has not yet been a successful attempt to use IgY antibodies to protect animals from Map. An effective method for the prevention or control of paratuberculosis would be a significant breakthrough in the fight against one of the most economically important ruminant diseases worldwide. The production of such an effector would also represent a great economic opportunity. Also, the preparation and effective use of IgY antibodies against PFO and NetB toxins of the bacterium Clostridium perfringens will be a completely new and original contribution to the science, as this has not yet been achieved according to available data in the literature. With the use of the inactivation antibodies, it will be possible to study more successfully the mechanism of toxin activity and determine their site of action in the host. In addition, for the disease prevention, the development of this non-invasive method, which currently has practically not been used yet for the disease control in the EU, is an extremely important contribution to the development of the field of profession. Namely, it can be expected in the near future that the importance of using alternatives to antibiotic therapy will greatly increase due to the increased resistance of bacteria to antimicrobial drugs. Thus, the experiences that will be gained during the proposed project will also be important in the development and implementation of this technology for the control of other diseases.
Significance for the country
The production and use of antibodies derived from chicken eggs has been gaining in importance in the recent years. Despite the fact that procedures for the prevention or treatment of various bacterial and viral gastrointestinal diseases of domestic animals have already been developed, there has not yet been a successful attempt to use IgY antibodies to protect animals from Map. An effective method for the prevention or control of paratuberculosis would be a significant breakthrough in the fight against one of the most economically important ruminant diseases worldwide. The production of such an effector would also represent a great economic opportunity. Also, the preparation and effective use of IgY antibodies against PFO and NetB toxins of the bacterium Clostridium perfringens will be a completely new and original contribution to the science, as this has not yet been achieved according to available data in the literature. With the use of the inactivation antibodies, it will be possible to study more successfully the mechanism of toxin activity and determine their site of action in the host. In addition, for the disease prevention, the development of this non-invasive method, which currently has practically not been used yet for the disease control in the EU, is an extremely important contribution to the development of the field of profession. Namely, it can be expected in the near future that the importance of using alternatives to antibiotic therapy will greatly increase due to the increased resistance of bacteria to antimicrobial drugs. Thus, the experiences that will be gained during the proposed project will also be important in the development and implementation of this technology for the control of other diseases.
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