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Projects / Programmes source: ARRS

Use of probiotics to control contamination of poultry farms with campylobacters and salmonella

Research activity

Code Science Field Subfield
4.04.02  Biotechnical sciences  Veterinarian medicine  Animal pathology and epizootiology 

Code Science Field
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 

Code Science Field
4.03  Agricultural and Veterinary Sciences  Veterinary science 
Keywords
Campylobacter sp., Salmonella Infantis, poultry, probiotic bacteria, gut microbiota, food safety
Evaluation (rules)
source: COBISS
Researchers (23)
no. Code Name and surname Research area Role Period No. of publications
1.  28448  PhD Jana Avberšek  Veterinarian medicine  Researcher  2019 - 2022  106 
2.  14880  PhD Igor Gruntar  Veterinarian medicine  Researcher  2019 - 2022  107 
3.  30755  PhD Sandra Janežič  Microbiology and immunology  Researcher  2019 - 2022  134 
4.  24640  MSc Rahela Juršič Cizerl  Veterinarian medicine  Researcher  2019 - 2020  50 
5.  38142  PhD Tanja Knific  Veterinarian medicine  Junior researcher  2019 - 2020  61 
6.  24643  MSc Boštjan Kostanjevec  Animal production  Researcher  2020 - 2022  18 
7.  23320  PhD Uroš Krapež  Veterinarian medicine  Researcher  2019 - 2022  164 
8.  24296  PhD Darja Kušar  Veterinarian medicine  Researcher  2019 - 2022  177 
9.  37544  PhD Aleksander Mahnič  Microbiology and immunology  Researcher  2021 - 2022  56 
10.  11879  PhD Jasna Mičunovič  Veterinarian medicine  Technician  2019 - 2022  84 
11.  54568  Sabina Mlakar    Technician  2020 - 2022 
12.  11133  PhD Matjaž Ocepek  Veterinarian medicine  Principal Researcher  2019 - 2022  460 
13.  38144  PhD Bojan Papić  Veterinarian medicine  Researcher  2019 - 2022  82 
14.  24612  PhD Mateja Pate  Veterinarian medicine  Researcher  2019  273 
15.  24598  PhD Tina Pirš  Veterinarian medicine  Technician  2019 - 2022  82 
16.  12278  PhD Maja Rupnik  Microbiology and immunology  Researcher  2019 - 2022  639 
17.  22446  PhD Brigita Slavec  Veterinarian medicine  Researcher  2019 - 2022  184 
18.  25606  PhD Brigita Tepuš  Control and care of the environment  Researcher  2019 - 2022  67 
19.  33511  PhD Valerija Tkalec  Microbiology and immunology  Researcher  2019 - 2020  49 
20.  24647  Brigita Vindiš Zelenko  Control and care of the environment  Researcher  2019 - 2020  14 
21.  37161  Tanja Vrabič  Microbiology and immunology  Technician  2019 - 2020 
22.  33447  Urška Zajc  Veterinarian medicine  Technician  2019 - 2022  72 
23.  08023  PhD Olga Zorman Rojs  Veterinarian medicine  Researcher  2019 - 2022  403 
Organisations (3)
no. Code Research organisation City Registration number No. of publications
1.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  9,696 
2.  2106  PERUTNINA PTUJ reja perutnine, proizvodnja krmil, perutninskega mesa in izdelkov, trgovina in storitve d.o.o. (Slovene)  Ptuj  5141966  53 
3.  3334  National Laboratory of Health, Environment and Foodstaffs  Maribor  6489087  4,064 
Abstract
Salmonella spp. and Campylobacter spp. are the main causative agents of bacterial foodborne enteric diseases in humans worldwide. Poultry is an important reservoir of these bacteria and the main source of infections for humans is the poultry meat. Several approaches are used in veterinary medicine aiming to reduce the colonization of poultry gut with these bacteria and, by following the good hygiene practices, to prevent the faecal contamination of poultry carcasses in slaughterhouses. The biosafety measures to prevent the introduction of salmonellae and campylobacters into the farms are not feasible in the countries with a high prevalence of these pathogens. An alternative approach is the use of probiotics in poultry production, which are added to feed and water or into the farm environment. Probiotics inhibit the pathogenic microbes and provide a favourable equilibrium between the commensals and pathogens in the animal gut. In addition, probiotics improve the absorption of nutrients and therefore the body weight gain of animals and the gut immunity. The use of probiotics also affects the meat quality and safety which is necessary from the viewpoint of assuring safe food for the consumers. Slovenia has a high prevalence of campylobacters in broiler flocks (approx. 70 %). As in other European countries, the prevalence of S. Infantis is increasing both in humans and in poultry. S. Infantis is often multidrug resistant and is able to persist in the farm environment due to biofilm formation. The effect and influence of probiotic preparations on broiler gut microbiota and the host-pathogen interactions are poorly understood. By using the metagenomic approach, the aim of this project is to study the gut microbiota of broilers from the flocks contaminated by campylobacters and/or S. Infantis in relation to different application regimens of two probiotic preparations. The objective is to investigate if the intestinal microbiota influences the susceptibility of broilers for the colonization with campylobacters and salmonellae from the farm environment and to study the role of probiotics in the regulation of this colonization. The control of these pathogens, which do not cause any disease in poultry, is urgent in the scope of food safety. Thus, the influence of probiotics will be evaluated also in the slaughterhouse by enumeration of campylobacters and S. Infantis on the carcasses of broilers from the flocks receiving the probiotics and from the flocks without them. New knowledge about the influence of probiotics on the chicken gut microbiota and on the presence of campylobacters and S. Infantis in the chicken intestinal tract and the carcasses will be used for the preparation of recommendations for the poultry industry. These recommendations will include a decrease in the use of antibiotics, leading to a decreased danger for the emergence of resistant strains in order to produce safe and quality food. The influence of probiotics on S. Infantis will represent an original contribution to the science.
Significance for science
The results obtained within this project will provide novel insights regarding the influence of probiotics on the composition of the gut microbiota of broilers and the presence of campylobacters and S. Infantis in the animal feces and on the carcasses of broilers after slaughter. Consequently, a possible direct beneficial effect of probiotics on the food safety will be confirmed, which has been poorly studied until now. S. Infantis is the most prevalent Salmonella serovar in broiler flocks and in poultry meat and is on the rise in the EU and worldwide. The influence of probiotics on the prevalence of S. Infantis in poultry will be an important original contribution to science as there are no publications in this field yet.   This project is in agreement with the following two paragraphs of the call for project proposal: JPI FACCE (Agriculture, Food Security and Climate Change) One of the main concerns of poultry production is the prevention of diseases in poultry flocks and the production of safe – microbiologically unquestionable food. With the use of probiotics, which would be added to the animal drinking water and feed and directly to the farm environment, the project aims to prevent the occurrence of pathogenic bacteria in poultry flocks and thus the contamination of poultry meat. By reducing the costs in the poultry industry, the production of competitive, high-quality and safe food for the consumer would be ensured.   JPI AMR (Antimicrobial Resistance) An important cost in the poultry production is the use of antibiotics, which also represents a risk for the development of antibiotic-resistant bacterial strains. In addition, the employed antimicrobial agents are deposited in the environment through animal excretions, which can also pose a threat to humans. Interactions between bacteria and antibiotics in the environment can lead to the development of resistant bacterial populations that can be transmitted from the environment into the food chain. It is expected that the use of probiotics will alter the intestinal microbiota of chickens in such a manner that the animals will be less susceptible to pathogenic bacteria. Consequently, this would lead towards decreasing the consumption of antibiotics and reducing the risk of development of the antimicrobial resistance.
Significance for the country
The results obtained within this project will provide novel insights regarding the influence of probiotics on the composition of the gut microbiota of broilers and the presence of campylobacters and S. Infantis in the animal feces and on the carcasses of broilers after slaughter. Consequently, a possible direct beneficial effect of probiotics on the food safety will be confirmed, which has been poorly studied until now. S. Infantis is the most prevalent Salmonella serovar in broiler flocks and in poultry meat and is on the rise in the EU and worldwide. The influence of probiotics on the prevalence of S. Infantis in poultry will be an important original contribution to science as there are no publications in this field yet.   This project is in agreement with the following two paragraphs of the call for project proposal: JPI FACCE (Agriculture, Food Security and Climate Change) One of the main concerns of poultry production is the prevention of diseases in poultry flocks and the production of safe – microbiologically unquestionable food. With the use of probiotics, which would be added to the animal drinking water and feed and directly to the farm environment, the project aims to prevent the occurrence of pathogenic bacteria in poultry flocks and thus the contamination of poultry meat. By reducing the costs in the poultry industry, the production of competitive, high-quality and safe food for the consumer would be ensured.   JPI AMR (Antimicrobial Resistance) An important cost in the poultry production is the use of antibiotics, which also represents a risk for the development of antibiotic-resistant bacterial strains. In addition, the employed antimicrobial agents are deposited in the environment through animal excretions, which can also pose a threat to humans. Interactions between bacteria and antibiotics in the environment can lead to the development of resistant bacterial populations that can be transmitted from the environment into the food chain. It is expected that the use of probiotics will alter the intestinal microbiota of chickens in such a manner that the animals will be less susceptible to pathogenic bacteria. Consequently, this would lead towards decreasing the consumption of antibiotics and reducing the risk of development of the antimicrobial resistance.
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