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Projects / Programmes source: ARIS

Razvoj metode PCR za ugotavljanje bakterij Listeria monocytogenes v zivilih (Slovene)

Research activity

Code Science Field Subfield
4.02.04  Biotechnical sciences  Animal production  Processing of animal raw materials 

Code Science Field
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
T430  Technological sciences  Food and drink technology 
Keywords
Listeria monocytogenes, detection, food, PCR
Evaluation (rules)
source: COBISS
Researchers (6)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  17799  Ivana Avbelj    Researcher  2000 - 2002 
2.  11548  PhD Barbka Jeršek  Animal production  Head  2000 - 2002  423 
3.  06020  MSc Nataša Klun  Microbiology and immunology  Researcher  2000 - 2002  70 
4.  19584  Eva Modec    Researcher  2000 - 2002 
5.  19583  Tatjana Rupel  Microbiology and immunology  Researcher  2000 - 2002  47 
6.  07030  PhD Sonja Smole - Možina  Animal production  Researcher  2000 - 2002  1,116 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  67,219 
Abstract
The objective of the project is to develop rapid and reliable PCR-based method for the detection of Listeria monocytogenes in food. The PCR-based method will have equivalent analytical parameters as the standard method (ISO 11290): detection limit of 100 cfu / 25 g, 0 % false negativity and false positivity, and will require 2 - 4 days less time. These parameters will be achieved by utilizing a part of standard sample-processing and enrichment methodologies, and the optimized post-enrichment linked to PCR. The developed method will be optimized using model samples, and evaluated analyzing a wide range of authentic samples, in cooperation with food hygiene control laboratory.
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