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Projects / Programmes source: ARIS

Toxinology

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Periods
January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
P310  Natural sciences and mathematics  Proteins, enzymology 
P320  Natural sciences and mathematics  Nucleic acids, protein synthesis 
P340  Natural sciences and mathematics  Lipids, steroids, membranes 
B220  Biomedical sciences  Genetics, cytogenetics 
B330  Biomedical sciences  Palaeozoology, phylogeny 
B470  Biomedical sciences  Physiology 
Evaluation (rules)
source: COBISS
Evaluation of bibliographic research performance indicators according to ARIS methodology
Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases
source: WoS source: Scopus source: COBISS
Researchers (16)
no. Code Name and surname Research area Role Period No. of publications
1.  20942  PhD Benjamin Gorinšek  Biochemistry and molecular biology  Researcher  2001 - 2003  17 
2.  01083  PhD Franc Gubenšek  Biochemistry and molecular biology  Head  2001 - 2003  535 
3.  15968  PhD Gabriela Ivanovski  Biochemistry and molecular biology  Researcher  2001 - 2003  34 
4.  15587  Igor Koprivec    Researcher  2001 - 2003  0 
5.  07673  PhD Dušan Kordiš  Biochemistry and molecular biology  Researcher  2001 - 2003  215 
6.  00412  PhD Igor Križaj  Biochemistry and molecular biology  Researcher  2001 - 2003  726 
7.  18802  PhD Adrijana Leonardi  Biochemistry and molecular biology  Researcher  2001 - 2003  156 
8.  20213  PhD Toni Petan  Biochemistry and molecular biology  Researcher  2001 - 2003  177 
9.  19422  Polonca Pirš    Researcher  2001 - 2003  0 
10.  04570  PhD Jože Pungerčar  Biochemistry and molecular biology  Researcher  2001 - 2003  320 
11.  15813  PhD Boris Rogelj  Neurobiology  Researcher  2001 - 2002  412 
12.  14315  PhD Ana Schweiger  Biochemistry and molecular biology  Researcher  2001 - 2002  62 
13.  21553  PhD Jernej Šribar  Biochemistry and molecular biology  Researcher  2001 - 2003  108 
14.  22326  MSc Anita Štrekelj  Biochemistry and molecular biology  Researcher  2002 - 2003  3 
15.  20214  PhD Nina Vardjan  Neurobiology  Researcher  2001 - 2003  267 
16.  15640  PhD Vera Župunski  Biochemistry and molecular biology  Researcher  2001 - 2003  185 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0106  Jožef Stefan Institute  Ljubljana  5051606000  90,742 
Abstract
Investigations of porcine cerebral cortex neuronal membrane receptors of ammodytoxin have shown that there are two proteins that bind ammodytoxin. The large one R180, a 180 kDa protein, is related to PLA2 receptor from rabbit sceletal muscle and also similar to slightly larger (200 kDa) PLA2 receptor found in porcine liver. Besides neurotoxic PLA2s it binds also non-toxic enzymes. The other, 25 kDa high affinity receptor found to be present in one of the intracelular membranes of porcine cerrebral cortex binds strongly only particular neurotoxic PLA2s. It differs in pharmacological and structural properties from all other PLA2 receptors identified so far. Some residues involved in toxicity of ammodytoxin were identified by site-directed mutagenesis. They are located in the C-terminal region of the molecule. Exchange of Tyr115 and Ile 116 for two Lys resulted in a drastic, two orders of magnitude, drop of lethality, whereas enzymatic activity was decreased only twofold. The two hydrophobic residues are apparently important for the high toxicity of ammodytoxin A. Basic Lys residues at positions 108 and 111 were substituted with two Asn without having an effect on the toxicity. This means that in these positions the basic residues are not required for the toxic effect of ammodytoxin. Evolutionary investigations of retrotransposon BovB-LINE has shown that this transposable element was horizontally transmitted from ancestral Boidae snakes to ancestor of ruminants about 45 million years ago. This was the first discovery of a horizontal transfer of DNA between two so distant vertebrate classes therefore it attracted considerable interest among evolutionary biologists, which resulted in invitations to present our results at several conferences in Europe and in USA.
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