Projects / Programmes
Development of molecular tools for conservation of identity and genetic diversity of marble trout
| Code |
Science |
Field |
Subfield |
| 4.06.00 |
Biotechnical sciences |
Biotechnology |
|
| Code |
Science |
Field |
| B220 |
Biomedical sciences |
Genetics, cytogenetics |
| B280 |
Biomedical sciences |
Animal ecology |
| B320 |
Biomedical sciences |
Systematic zoology, taxonomy, zoogeopraphy |
| B402 |
Biomedical sciences |
Aquaculture, pisciculture |
marble trout, genetic markers, hybridisation, polymorphism, conservation genetic
Researchers (8)
Organisations (3)
Abstract
The marble trout (Salmo marmoratus), a freshwater fish endemic to the Adriatic drainage, is an endangered species because of hybridisation resulting from intensive stocking with allochthonous brown trout (Salmo trutta). A project for rehabilitating marble trout in Slovenia has been undertaken. It is based on the replacement of a population of introduced and introgressed fish by population of non-intogressed (=pure) fish of native species. Up to now eight stations with pure marble trout populations were found. These populations are used for the creation of a pure marble trout strain for restocking. In order to ensure the long-term survival of reintroduced marble trout it is vitally to sustain its genetic variability. However, almost nothing is known about marble trout genetic diversity so far. In order to answer the question whether there is a gene pool with suitable gene forms within pure marble trout populations, sufficient to maintain their adaptiveness in areas to be reintroduced, their intra- and inter-population genetic polymorphism will be studied. In the next stage, a genetic structure of populations from several hybridisation zones will be surveyed. By comparing both sets of collected data we will try to determine which populations of pure marble trout would be the most appropriate stocking material for repopulation of particular hybridisation zones. This knowledge will also give a better insight into the circumstances which took place in the marble trout demography before being changed by human activities.Genetic polymorphism of marble trout and its hybrids will be studied within three convenient and informative genetic systems: microsatellite DNA, random amplified polymorphic DNA (RAPD) and mitochondrial DNA, which are able to detect polymorphisms and allow the identification of informative genealogic characters among even closely related species and populations.
