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Projects / Programmes source: ARIS

Concentration and purification of plant viruses on monolithic supports (CIM®)

Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
Keywords
chromatography-optimization of CIM monolithic support , CIM, monolith, viruses
Evaluation (rules)
source: COBISS
Researchers (12)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  12667  PhD Miloš Barut  Chemistry  Researcher  2002 - 2004  206 
2.  18343  Aleš Blatnik    Researcher  2002 - 2004  48 
3.  12027  PhD Karmen Čuček  Chemistry  Researcher  2002 - 2004  39 
4.  17278  MSc Janez Jančar  Chemical engineering  Researcher  2002 - 2004  61 
5.  21577  PhD Petra Kramberger  Chemical engineering  Researcher  2002 - 2004  94 
6.  15489  PhD Irena Mavrič Pleško  Plant production  Researcher  2001 - 2002  374 
7.  16327  PhD Matjaž Peterka  Biotechnology  Researcher  2002 - 2004  218 
8.  10263  PhD Nataša Petrovič  Biotechnology  Researcher  2002 - 2004  161 
9.  12728  PhD Aleš Podgornik  Chemical engineering  Researcher  2002 - 2004  709 
10.  05229  PhD Maja Ravnikar  Biotechnology  Researcher  2002 - 2004  1,360 
11.  00779  PhD Aleš Štrancar  Chemical engineering  Head  2002 - 2004  479 
12.  21576  MSc Marjeta Štrancar  Biotechnology  Researcher  2002 - 2004 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,315 
2.  1655  Sartorius BIA Separations L.t.d., Separations technology company  Ajdovščina  1319612  1,189 
Abstract
Purification and concentration of plant viruses by chromatography would shorten the purification and/or concentration of different viruses. This area is expected to develop very fast in next few years, especially because of the potential usage of recombinant viruses as vectors in gene therapy. Chromatographic supports applicable for fast and efficient purification will become indispensable. Our preliminary tests showed that monolithic CIM® supports have a lot of advantages in comparison to other classical chromatographic supports, above all they enable fast and efficient concentration and separation.This project will serve us as a study of virus behavior on chromatographic supports. Because of the safety reasons our model will be plant viruses, which represent no hazard to environment or personnel. First we will propagate model plant virus in a suitable propagation plant, remove the plant material clarification and continue purification and concentration on monolithic chromatographic support (CIM®). Chromatographic conditions will be optimized as well as the structure of the pores of the chromatographic support. We will use ion exchangers, and where possible, also affinity monolithic CIM® columns.The results of this project will serve as a basis of preparing different products for purification and concentration of different plant viruses. The applicability of these products will be important especially for improvement of detection methods (e.g. ELISA, PCR) of viruses in different samples, above all in samples with low concentration of viruses (e.g. potable water). The knowledge which we will gain will be used for development of new monolithic CIM® supports and the methods for purification of virus vectors. Also we will use it for the development of supports and methods for improvement of detection methods of human viruses, especially in potable water.
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