Projects / Programmes
Industrial heterologous enzymes: bioprocess development and optimization
| Code |
Science |
Field |
Subfield |
| 4.06.00 |
Biotechnical sciences |
Biotechnology |
|
| Code |
Science |
Field |
| T490 |
Technological sciences |
Biotechnology |
industrial heterologous enzymes, expression system, Streptomyces rimosus, recombinant DNA technology, bioprocess, enzyme production
Researchers (9)
Organisations (1)
Abstract
Industrial enzymes are very interesting biotechnological products; they are also an essential raw material for our industrial partner TKI Hrastnik. The market monopoly on theses products is imposing an increasing economic burden. This is the inducement for our project proposal, which focuses on the development of a suitable industrial bioprocess (expression system) for the production of heterologous industrial enzymes by the bacterial host Streptomyces rimosus. Numerous expression systems (hosts) are described in the literature but only a few are industrially useful. The bacterium S. rimosus is an industrial producer of oxytetracycline and it is generally regarded as safe (GRAS). Advantages of S. rimosus are its fast growth, the culture can reach high cell density, it is easily to transformable and it can secrete products out of the cell. Good understanding of the genetics and the physiology of the S. rimosus host and the availability of industrial media and bioprocess parameters for this potential host constitute a solid basis for the development of an expression system for heterologous protein production. For the successful realization of the proposed project we also count with the required genetic tools, the equipment and the necessary knowledge. With this as our starting point we will develop and optimize the genetic tools needed for the introduction and expression of target heterologous enzymes. Optimization of the media and key bioprocess parameters for the production of the selected enzymes at laboratory scale will follow. Towards the final stages of the project, the process will be scaled up to a bioreactor. The final aim of the proposed project is to develop a bioprocess, which will enable TKI Hrastnik to set up its own technology for the production of enzymes, thus increasing the quality of their final product and overall competitiveness.
