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Projects / Programmes source: ARIS

Industrial heterologous enzymes: bioprocess development and optimization

Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
Keywords
industrial heterologous enzymes, expression system, Streptomyces rimosus, recombinant DNA technology, bioprocess, enzyme production
Evaluation (rules)
source: COBISS
Researchers (9)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  27641  PhD Tjaša Danevčič  Biotechnology  Researcher  2006 - 2008  183 
2.  21392  PhD Štefan Fujs  Biotechnology  Researcher  2006 - 2008  87 
3.  18511  PhD Polona Jamnik  Biotechnology  Researcher  2006 - 2008  904 
4.  25521  PhD Urška Lešnik  Biotechnology  Junior researcher  2006 - 2008  31 
5.  05993  PhD Ines Mandić-Mulec  Biotechnology  Researcher  2006 - 2008  639 
6.  13542  PhD Hrvoje Petković  Biotechnology  Head  2005 - 2008  300 
7.  10873  PhD Nataša Poklar Ulrih  Chemistry  Researcher  2006 - 2008  828 
8.  04001  PhD Peter Raspor  Microbiology and immunology  Researcher  2006 - 2008  1,898 
9.  13005  PhD David Stopar  Plant production  Researcher  2006 - 2008  469 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,215 
Abstract
Industrial enzymes are very interesting biotechnological products; they are also an essential raw material for our industrial partner TKI Hrastnik. The market monopoly on theses products is imposing an increasing economic burden. This is the inducement for our project proposal, which focuses on the development of a suitable industrial bioprocess (expression system) for the production of heterologous industrial enzymes by the bacterial host Streptomyces rimosus. Numerous expression systems (hosts) are described in the literature but only a few are industrially useful. The bacterium S. rimosus is an industrial producer of oxytetracycline and it is generally regarded as safe (GRAS). Advantages of S. rimosus are its fast growth, the culture can reach high cell density, it is easily to transformable and it can secrete products out of the cell. Good understanding of the genetics and the physiology of the S. rimosus host and the availability of industrial media and bioprocess parameters for this potential host constitute a solid basis for the development of an expression system for heterologous protein production. For the successful realization of the proposed project we also count with the required genetic tools, the equipment and the necessary knowledge. With this as our starting point we will develop and optimize the genetic tools needed for the introduction and expression of target heterologous enzymes. Optimization of the media and key bioprocess parameters for the production of the selected enzymes at laboratory scale will follow. Towards the final stages of the project, the process will be scaled up to a bioreactor. The final aim of the proposed project is to develop a bioprocess, which will enable TKI Hrastnik to set up its own technology for the production of enzymes, thus increasing the quality of their final product and overall competitiveness.
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