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Projects / Programmes source: ARIS

Effect of different cultivation mode and conditions on nucleotide accumulation (RNA) in yeast cell

Research activity

Code Science Field
T490  Technological sciences  Biotechnology 
T430  Technological sciences  Food and drink technology 
Keywords
cultivation/biomass/yeasts/RNA/5''-nucleotides
Evaluation (rules)
source: COBISS
Researchers (5)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  07469  PhD Martin Batič  Biotechnology  Head  1999  193 
2.  07495  MSc Darinka Blažica  Plant production  Researcher  1998 - 1999 
3.  11222  PhD Boris Kovač  Biotechnology  Researcher  1997 - 1999  194 
4.  01344  PhD Hermina Leskovšek Šefman  Chemistry  Researcher  1997 - 1999  129 
5.  04001  PhD Peter Raspor  Microbiology and immunology  Researcher  1997 - 1999  1,896 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0106  Jožef Stefan Institute  Ljubljana  5051606000  90,046 
2.  0197  Mlinotest Živilska industrija d.d. (Slovene)  Ajdovščina  5132061000  205 
Abstract
Yeast biomass production with high content of RNA by Saccharomyces cerevisiae ZIM-753 and Candida utilis ZIM-70 was lasting in aerobic batch conditions included selection and optimization of medium, and bioprocess parameters. According to results of screening methods of four C:N ratio (5:1; 10:1; 25:1; 50:1) and starting pH (2, 4, 6, 8) of media on the shaker the followed bioprocess parameters were selected for Saccharomyces cerevisiae: media with C:N ratio 5:1, starting pH 4.0, bioproces duration 15 hours, temperature 29°C, air flow 2 vvm and 250 rpm. In Candida utilis the media C:N ratio was 10:1, starting pH 6.0, and bioproces duration 12 hours. During the bioprocess in bioreactor the ''''on line'''' and ''''off line'''' measurements of pH, dry biomass, RNA, proteins, O2, CO2, glucose, nitrogen and organic acids were followed. In bioprocess of growth of Saccharomyces cerevisiae ZIM-753 the biomass concentration was 6.25 g/L and specific growth rate 0.24 h-1. The ratio of RNA and proteins in the yeast biomass was 7.3% and 39%, respectively. The concentraction of acetic acid and citric acid attained values 0.74 g/L and 0.1 g/L. The productivity for RNA production was obtained value 4.8 mg RNA/ g biomas h. In the case of Candida utilis ZIM-70 the concentration of biomass reached value 10.6 g/L at specific growth rate 0.4 h-1. RNA and proteins content in yeast biomass reached 4,9% and 34% while RNA productivity was in this case 4.1 mg of RNA/g biomas h.
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