Projects / Programmes
Interactions between nanoparticles with different surfaces and model biological systems
Code |
Science |
Field |
Subfield |
1.03.01 |
Natural sciences and mathematics |
Biology |
Zoology and zoophysiology |
Code |
Science |
Field |
B360 |
Biomedical sciences |
Animal physiology |
Code |
Science |
Field |
1.06 |
Natural Sciences |
Biological sciences |
nanoparticles, nanotechnologies, bio-nano interactions, nanotoxicity, cellular internalisation, tissue assimilation
Researchers (18)
Organisations (6)
no. |
Code |
Research organisation |
City |
Registration number |
No. of publicationsNo. of publications |
1. |
0105 |
National Institute of Biology |
Ljubljana |
5055784 |
13,278 |
2. |
0381 |
University of Ljubljana, Faculty of Medicine |
Ljubljana |
1627066 |
48,238 |
3. |
0382 |
University of Ljubljana, Faculty of Health Sciences |
LJUBLJANA |
1627155 |
14,413 |
4. |
0481 |
University of Ljubljana, Biotechnical Faculty |
Ljubljana |
1626914 |
66,333 |
5. |
0792 |
University of Ljubljana, Faculty of Civil and Geodetic Engineering |
Ljubljana |
1626981 |
25,725 |
6. |
1538 |
University of Ljubljana, Faculty of Electrical Engineering |
Ljubljana |
1626965 |
27,771 |
Abstract
The research proposed in the project is based on the review published by Dawson in Nature Nanotechnology (2009). He commented on the interesting results obtained by Granick and his colleagues published in the Proceedings of the National Academy of Sciences USA where they provided some new considerations on interactions between nanoparticles and biological membranes. Granick and his colleagues reported that nanoparticles can actively modulate the phase structure of lipid membranes so that the stiffness of the membrane is not uniform. This variation in stiffness is functionally important for material and sensor applications, and the findings could also have broader implications for an understanding of nanoparticle-cell interactions and related safety issues (Dawson et al. 2009). The possibility was discussed of directed physical interactions between nanoparticles and cell membranes.
The objective of the proposed project is to design experiments in which nanoparticle- lipid membranes will be studied. The proposed project is composed of three parts. In the first part microscopic and spectroscopy techniques as well as computer assisted microscopy will be used to study lipid membrane - nanoparticle interactions. We expect that the intensity and type of interactions will depend on primary characteristics of particles and characteristics of a particle suspension (secondary characteristics). In the second part, results of the first part will be validated in vivo. A model invertebrate organism will be exposed to nanoparticles via food or via single oral gavage. After exposure, three types of biomarkers will be measured. These are biomarkers for physical interactions between cell membrane and particles, biomarkers for altered metabolic activity and biomarkers for oxidative stress. In addition, FTIR analyses of digestive gland epithelium will be analyzed in order to assess a molecular fingerprint in non-exposed animals versus nanoparticle fed animals. In this part we expect to confirm the hypothesis that particles which affected artificial lipid membranes have also the potential to affect cell membrane in vivo. Further, we will test the hypothesis here that nanoparticles which do not affect artificial lipid membranes have lower potential to affect cell membrane in vivo. We expect that data obtained with selected biomarkers and FTIR analyses will allow discussing whether nanoparticles first exert oxidative stress which subsequently leads to cell membrane destabilization or cell membrane is destabilized due to direct contact between nanoparticles and cells. In the third part, in vivo cellular internalization of some selected nanoparticles will be studied by x-ray based methods (PIXE, SR XRF, EDX-SEM analyses). Here we expect to reveal which characteristic of nanoparticles are responsible for cellular internalization of nanoparticles.
For our knowledge, there is no evidence in the literature on linking data on nanoparticle characteristics, experimental data on artificial lipid vesicles and in vivo realistic exposure. The project will start to fill this gap in knowledge.
The partnership of the leading research group in a large EU 7 FP (NanoValid) and two domestic centers of excellence (Co Nanocentre and Co NAMASTE) will provide the access to best possible characterized nanoparticle and topmost research equipment.
Kenneth A. Dawson, Anna Salvati, and Iseult Lynch. Nanotoxicology: Nanoparticles reconstruct lipids. Nature Nanotechnology, 4(2):84–85, 2009.
Significance for science
In this project we have provide experimental data on the effects of nanoparticles on lipid membranes. Since lipid membranes are major constituents of biological membranes we have focused on the effect of nanoparticles on the lipid component of the biological membrane. This was validated also in in vivo system. The key findings of the project are: - Nanoparticles are not internalised by intact cells - Nanoparticles enter the cells only when cell membrane is damaged - the effect of nanoparticles on lipid membranes is less pronounced as expected at the beginning of the project.
Significance for the country
With the rapid rise of the field of nanotechnology and the design and production of increasingly complex nanoscale materials, it has become ever more important to understand how the physical form and chemical composition of these materials interact synergistically to determine bio-nano interactions. During the development of any nanotechnological product for biomedical application, application in pharmacy food industry and similar, controlled interactions with biological systems have to be studied in order to reach the desired application. However, when a product is to be put on the market its potential side effects have to be known as well. The knowledge obtained in this proejkt is already dissiminated to two sloveian companies: Zlatara and Cinkarna Celje.
Most important scientific results
Annual report
2011,
2012,
2013,
final report,
complete report on dLib.si
Most important socioeconomically and culturally relevant results
Annual report
2011,
2012,
2013,
final report,
complete report on dLib.si