Projects / Programmes source: ARIS

Biokemijska in biofizikalno-kemijska karakterizacija naravnih snovi (Slovene)

Research activity

Code Science Field Subfield
4.03.07  Biotechnical sciences  Plant production  Technology of vegetal origin food 

Code Science Field
T430  Technological sciences  Food and drink technology 
P400  Natural sciences and mathematics  Physical chemistry 
naturally occuring substances, coligative properties, transport and volumetric properties, electrolytic conductivity, HPLC, enzymatic hydrolysis, spectroscopy
Evaluation (rules)
source: COBISS
Researchers (12)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  01522  PhD Veronika Abram  Biochemistry and molecular biology  Researcher  1998 - 2001  309 
2.  07028  PhD Helena Abramovič  Plant production  Researcher  1998 - 2001  255 
3.  17796  Ana Bučar    Researcher  1996 - 2001 
4.  15581  PhD Blaž Cigić  Biochemistry and molecular biology  Researcher  1996 - 2001  361 
5.  12484  MSc Andreja Gramc Livk  Biochemistry and molecular biology  Researcher  2000 - 2001  12 
6.  17797  Andreja Habjan    Researcher  2000 - 2001 
7.  00177  PhD Florijan Klofutar  Chemistry  Head  1998 - 2001  428 
8.  17798  Jana Martinuč    Researcher  2000 - 2001 
9.  09633  PhD Darja Rudan-Tasič  Chemistry  Researcher  2000 - 2001  135 
10.  15466  PhD Mihaela Skrt  Biotechnology  Researcher  1996 - 2001  158 
11.  08384  PhD Nataša Šegatin  Chemistry  Researcher  1999 - 2001  135 
12.  01647  PhD Marija Zelenik-Blatnik  Biochemistry and molecular biology  Researcher  1998 - 2001  134 
In the report the viscosities and apparent specific volumes of pectic acid and its’ lithium salt in aqueous solutions as well as some other solutes, e.g. poly(ethylene glycol)s, 1-octanol, 1-nonanol, 1-decanol and cyclohexanol in aqueous and nonaqueous media are given. The partial molar or partial specific volume of the solute and solvent and molar or specific excess volume of the solution were evaluated and interpreted in terms of solute-solute and solute-solvent interactions. The limiting partial molar volume of the solute was interpreted in terms of the scaled particle theory. The ''''solvent effect'''' on the partial molar volume of the solute was found to be due mainly to cavity formation and intermolecular dipersion forces. The intrinsic viscosity and the viscosity average molecular weight of the solute was calculated applying Eyring’s transition state theory. The thermodynamic functions of activation of viscous flow of binary mixtures i.e. the molar Gibbs free energy, entropy and enthalpy were estimated and interpreted in term of the relative effects of solute on the ground and transition state solvent. In addition, the optimal conditions of corn starch liquefaction and dextrinization using alpha-amylase from Bacillus subtilis to obtain maltodextrins with a dextrose equivalent of 12,9 - 23,5 were obtained. The pattern of action of B. subtilis alpha-amylase on native corn starch was monitored using high-pressure liquid cromatography (HPLC) and high-performance thin layer chromatography (HPTLC). It was possible to separate malto-oligosaccharides of up to 9 glucose units by HPLC and those of up to 12 glucose units by HPTLC, respectlively. The polyphenols from fresh leaves of Sempervivum tectorum were isolated using an extraction technique. After acidic hydrolysis of the oligomeric polyphenols, it was established by TLC, HPLC and FAB mass spectra that kaempferol was the unique aglycon of the three main oligomeric constituents of S.tectorum.
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