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Projects / Programmes source: ARRS

Self-assembled and advanced biopolymer coatings for microencapsulation of probiotics and starter cultures

Research activity

Code Science Field Subfield
4.03.00  Biotechnical sciences  Plant production   
Keywords
microbial microencapsulation; carrier design; biopolymers; self assembly; augmented fermentation; targeted delivery; product inhibition resistance
Evaluation (rules)
source: COBISS
Points
9,890.96
A''
1,674.56
A'
5,217.28
A1/2
6,736.71
CI10
12,193
CImax
678
h10
48
A1
33.25
A3
31.35
Data for the last 5 years (citations for the last 10 years) on September 30, 2022; A3 for period 2016-2020
Data for ARRS tenders ( 04.04.2019 – Programme tender, archive )
Database Linked records Citations Pure citations Average pure citations
WoS  623  14,163  12,340  19.81 
Scopus  628  15,408  13,485  21.47 
Researchers (19)
no. Code Name and surname Research area Role Period No. of publications
1.  39095  PhD Miha Bahun  Biochemistry and molecular biology  Junior researcher  2020 - 2022  30 
2.  11150  PhD Bojana Bogovič Matijašić  Animal production  Researcher  2020 - 2022  362 
3.  51167  Doris Bračič  Textile and leather  Researcher  2020 - 2022  21 
4.  24407  PhD Iztok Dogša  Biotechnology  Researcher  2020 - 2022  171 
5.  19753  PhD Lidija Fras Zemljič  Textile and leather  Researcher  2020 - 2022  467 
6.  25788  PhD Boštjan Genorio  Materials science and technology  Researcher  2020 - 2022  238 
7.  18511  PhD Polona Jamnik  Biotechnology  Researcher  2020 - 2022  869 
8.  17916  PhD Iztok Jože Košir  Chemistry  Researcher  2020 - 2022  495 
9.  24332  PhD Manja Kurečič  Materials science and technology  Researcher  2020 - 2022  206 
10.  25516  PhD Petra Mohar Lorbeg  Animal production  Researcher  2020 - 2022  98 
11.  37419  PhD Miha Ocvirk  Plant production  Researcher  2020 - 2022  134 
12.  29336  PhD Ilja Gasan Osojnik Črnivec  Chemistry  Principal Researcher  2020 - 2022  194 
13.  36376  PhD Diana Paveljšek  Animal production  Researcher  2020 - 2022  28 
14.  35476  PhD Olivija Plohl  Textile and leather  Researcher  2020 - 2022  86 
15.  10873  PhD Nataša Poklar Ulrih  Chemistry  Researcher  2020 - 2022  768 
16.  23417  PhD Iztok Prislan  Chemistry  Researcher  2020 - 2022  188 
17.  53547  Ksenija Rutnik  Chemistry  Junior researcher  2020 - 2022  13 
18.  15466  PhD Mihaela Skrt  Biotechnology  Researcher  2020 - 2022  138 
19.  51227  Luka Šturm  Biochemistry and molecular biology  Researcher  2020 - 2022  22 
Organisations (4)
no. Code Research organisation City Registration number No. of publications
1.  0103  University of Ljubljana, Faculty of Chemistry and Chemical Technology  Ljubljana  1626990  21,273 
2.  0416  Slovenian Institute of Hop Research and Brewing  Žalec  5051762000  3,903 
3.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  63,013 
4.  0795  University ob Maribor, Faculty of mechanical engineering  Maribor  5089638010  23,601 
Abstract
Microencapsulation involves coating or entrapping of a core material with a polymeric material to generate microspheres, microcapsules or micro aggregates in the size range of 1–1000 µm. This versatile technology has been used to encapsulate a wide array of products such as pharmaceuticals, flavours, volatile oils, plant extracts, enzymes and other functional compounds. In recent decades, this technology has also been applied to the area of microbial cell immobilization owing to its numerous advantages over other cell immobilization techniques. Due to the abundance of natural biopolymers of diverse surface and colloidal properties, as well as the ability to study their interactions at the molecular level, numerous opportunities are offered for dedicated development of advanced delivery systems for encapsulation of microorganisms, especially for biotechnological food processes and in development of probiotics intended for ingestion. The purpose of encapsulation of cells is strongly dependent on its intended end-use. For encapsulation of starter cultures used in various fermentation processes, the goal is to shorten the period of adaptation in the slurry, achieve high cell density within the carrier, improve the kinetics of the process, reduce the possibility of microbial contamination and allow for easier separation and reuse of biomass for the next batch. For the case of probiotics which are intended for ingestion, the objectives of encapsulation are different as the carrier provides protection during storage and digestion, as well as in more advanced applications acting as a delivery system to reach target parts of the digestive tract or other tissues. In the field of material science, a number of properties have been shown to provide the basis for linking of biopolymers and related molecules as natural building blocks to provide either hollow, or porous and non-porous structures with varying degrees of internal and external organization thus indicating a part of their encapsulating potential. In particular, polymers, such as structural components of the cell wall of plants (pectin) or algae (alginate) or starch and its derivatives offer numerous possibilities for the preparation of materials with controlled size, morphology, surface and structural properties. Our project will be based on thorough characterization of the respective natural substances (mostly plant derived polysaccharides and other plant-based components), and their use as building blocks for self-assembly or targeted organization. The acquired supramolecular structures will serve as stand-alone encapsulants, as well as dedicated structural templates for a wide variety of microbial encapsulation approaches in order to suit to products, which are intended for ingestion and / or for contact with foodstuffs. The selected carriers will be used for two markedly different end-use applications, i.e. (i) for encapsulation of yeasts for optimized ethanol production, (ii) and to improve gut retention and act as a prebiotic for our own isolates of lactobacillus probiotics. In both cases, we will determine the encapsulation efficiency parameters, the level of supported and sustained microbial activity, as well as at the cellular level, the capability of the encapsulation system for fermentation or targeted delivery of microorganisms. The research project will set out to reach beyond the established alginate hydrogel systems using plant derived materials with more precisely defined surface characteristics in order to study the overlying physical, chemical and biological mechanisms that govern the efficiency of the microencapsulation process. We will employ and extend recent advances in the field of material science in order to generate in depth knowledge to allow for the design of food safe encapsulation carriers, perform through characterisation and material screening, as well as assess the suitability of such microencapsulated biomass for tacking current specific field i
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