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Projects / Programmes source: ARIS

Biological diversity among two grapevine viruses and their role in plant

Research activity

Code Science Field Subfield
1.03.00  Natural sciences and mathematics  Biology   

Code Science Field
B310  Biomedical sciences  Physiology of vascular plants 
Keywords
biological diversity, viruses, grapevine, physiology, immunolocalisation, sequences
Evaluation (rules)
source: COBISS
Researchers (9)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  18343  Aleš Blatnik    Researcher  2004  49 
2.  12688  PhD Kristina Gruden  Biotechnology  Researcher  2004 - 2007  985 
3.  18344  Lidija Matičič    Technical associate  2005 - 2007  42 
4.  19119  PhD Mojca Milavec  Biotechnology  Researcher  2005 - 2007  318 
5.  10263  PhD Nataša Petrovič  Biotechnology  Researcher  2004 - 2006  161 
6.  18467  PhD Maruša Pompe Novak  Biotechnology  Researcher  2004 - 2007  291 
7.  05229  PhD Maja Ravnikar  Biotechnology  Head  2004 - 2007  1,369 
8.  24821  Dejan Štebih  Biochemistry and molecular biology  Technical associate  2006 - 2007  163 
9.  17107  PhD Irma Vuk  Biotechnical sciences  Researcher  2004 - 2007  90 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,256 
2.  1540  University of Nova Gorica  Nova Gorica  5920884000  14,063 
Abstract
The project proposal addresses the problem of variability in genome sequences among well established and wide-spread pathogenic viruses, and its biological role. We have chosen to investigate two grapevine viruses, Grapevine fan leaf virus (GFLV) and Grapevine rupestris stem pitting associated virus (GRSPaV). Both viruses have important economical impact in plant protection and phytosanitary selection of grapevine. GFLV causes severe disease fanleaf degeneration, and GRSPaV has been associated with the severe disease called rugose wood complex on grapevine. Both viruses are spread with planting material, and GFLV is naturally spread by its nematode vector Xiphinema index. There is no known natural vector of GRSPaV. Analyses of virus genomes showed that both viruses exist in nature as families of sequence variants. This indicates their natural biological variability. Preliminary analyses showed that several sequence variants of the same virus coexists in the same host plant, and that recombination between different variants can occur. The later could influence the dynamics of natural virus populations and consequently the spread and pathogenicity of the two viruses. The objectives are to 1) survey the natural variability (characterization of natural virus populations by analysis of selected genes), 2) studies of the dynamics of natural virus of GFLV and GRSPaV in Slovenia (studies of recombination events between different viral sequence variants by sequence analyses), and 3) investigate how natural variability and recombination events influence virus-host relationship (disease symptoms at the level of plants, tissues and cells). We will describe morphological characteristcs using immuno elctron microscopy on ultra thin sections.
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