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Projects / Programmes source: ARIS

Development of quantitative real-time PCR for virus determination after purification procedure using monolithic cromatographic supports (CIM®)

Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
Keywords
viruses, real-time PCR, monolithic cromatographic supports CIM(R)
Evaluation (rules)
source: COBISS
Researchers (15)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  12667  PhD Miloš Barut  Chemistry  Researcher  2004 - 2007  206 
2.  22614  PhD Jana Boben  Biotechnology  Researcher  2004 - 2007  82 
3.  24077  PhD Peter Brne  Materials science and technology  Researcher  2004 - 2007  28 
4.  12027  PhD Karmen Čuček  Chemistry  Researcher  2004 - 2007  39 
5.  12688  PhD Kristina Gruden  Biotechnology  Researcher  2004 - 2007  992 
6.  17278  Janez Jančar  Chemical engineering  Technical associate  2004 - 2007  61 
7.  21577  PhD Petra Kramberger  Chemical engineering  Researcher  2004 - 2007  94 
8.  18344  Lidija Matičič    Technical associate  2004 - 2007  42 
9.  23610  PhD Nataša Mehle  Biotechnology  Researcher  2004 - 2007  556 
10.  19119  PhD Mojca Milavec  Biotechnology  Researcher  2005 - 2007  318 
11.  16327  PhD Matjaž Peterka  Biotechnology  Researcher  2004 - 2007  226 
12.  10263  PhD Nataša Petrovič  Biotechnology  Researcher  2004 - 2007  161 
13.  12728  PhD Aleš Podgornik  Chemical engineering  Researcher  2004 - 2007  713 
14.  05229  PhD Maja Ravnikar  Biotechnology  Researcher  2004 - 2007  1,374 
15.  00779  PhD Aleš Štrancar  Chemical engineering  Head  2004 - 2007  499 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,463 
2.  1655  Sartorius BIA Separations L.t.d., Separations technology company  Ajdovščina  1319612  1,238 
Abstract
Isolation and concentration of viruses from plant material is time consuming and demanding process that could be shorten and simplify by using some chromatographic techniques. Previous experiments have shown that CIM(R) monolithic support is a chromatographic material that can be used for purification, separation and concentration of plant viruses. We were able to concentrate virus particles from extremely diluted samples in which viruses were undetectable by ELISA or PCR. According to results, CIM(R) monolithic supports can be used for detection of viruses in irrigation water, from were they can be concentrated and subsequently detected by serological or other method. Project that we present here is a logical continuation of previous work. Two model plant viruses will be used. For detection and quantification of model viruses concentrated on CIM(R) monolithic chromatographic supports we will develop highly sensitive real-time PCR method. Conditions for separations and concentrations of model viruses on CIM(R) supports will be optimized. If needed, CIM supports will also be optimized. We will use the procedures for detection and quantification of viruses in rivers and lakes. Application of accurate molecular detection methods will significantly contribute to the knowledge about CIM(R) monolithic support properties and will help to describe a nature of virus-support interaction. Understanding of these interactions will enable application of CIM (R) supports on the other fields of virology.
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