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Projekti / Programi vir: ARRS

Izcedne talne vode kot neizkoriščen vir informacij o biotski raznovrstnosti tal

Raziskovalna dejavnost

Koda Veda Področje Podpodročje
4.01.00  Biotehnika  Gozdarstvo,  lesarstvo in papirništvo   

Koda Veda Področje
4.01  Kmetijske vede in veterina  Kmetijstvo, gozdarstvo in ribištvo 
Ključne besede
biodiverziteta tal, rabe tal, lizimetri, izcedne vode, voda, okoljska DNA, metabarkodiranje, standardizacija postopka
Vrednotenje (pravilnik)
vir: COBISS
Upoš. tč.
13.151,6
A''
1.464,5
A'
6.024,84
A1/2
8.340,43
CI10
21.352
CImax
2.190
h10
66
A1
44,44
A3
45,82
Podatki za zadnjih 5 let (citati za zadnjih 10 let) na dan 03. december 2022; A3 za obdobje 2016-2020
Podatki za razpise ARRS ( 04.04.2019 - Programski razpis, arhiv )
Baza Povezani zapisi Citati Čisti citati Povprečje čistih citatov
WoS  840  24.485  21.434  25,52 
Scopus  859  26.884  23.593  27,47 
Raziskovalci (39)
št. Evidenčna št. Ime in priimek Razisk. področje Vloga Obdobje Štev. publikacij
1.  50812  Katarina Belcijan  Biotehnologija  Raziskovalec  2021 - 2022  19 
2.  15493  dr. Matjaž Čater  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  286 
3.  52663  Rok Damjanić    Tehnični sodelavec  2021 - 2022  129 
4.  27641  dr. Tjaša Danevčič  Biotehnologija  Raziskovalec  2021 - 2022  162 
5.  19262  Svetlana Gogič Knežić    Tehnični sodelavec  2021 - 2022 
6.  25974  dr. Cene Gostinčar  Biotehnologija  Raziskovalec  2021 - 2022  287 
7.  54916  Anton Govednik  Rastlinska produkcija in predelava  Raziskovalec  2021 - 2022 
8.  21242  dr. Tine Grebenc  Rastlinska produkcija in predelava  Vodja projekta/programa  2021 - 2022  449 
9.  05935  dr. Nina Gunde-Cimerman  Biotehnologija  Raziskovalec  2021 - 2022  1.165 
10.  28855  Melita Hrenko    Tehnični sodelavec  2021 - 2022 
11.  32513  dr. Maja Jan  Biologija  Raziskovalec  2021 - 2022  78 
12.  17424  Franc Kljun    Tehnični sodelavec  2021 - 2022  49 
13.  32058  dr. Marjeta Konec  Biologija  Raziskovalec  2021 - 2022  52 
14.  06960  dr. Ivan Kos  Biologija  Raziskovalec  2021 - 2022  544 
15.  22492  dr. Barbara Kraigher  Biologija  Raziskovalec  2021 - 2022  107 
16.  07127  dr. Hojka Kraigher  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  1.290 
17.  50798  dr. Bor Krajnc  Varstvo okolja  Raziskovalec  2021 - 2022  62 
18.  39089  Žan Kuralt  Biologija  Raziskovalec  2021 - 2022  61 
19.  15108  dr. Lado Kutnar  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  763 
20.  21581  dr. Irena Maček  Biologija  Raziskovalec  2021 - 2022  127 
21.  05993  dr. Ines Mandić-Mulec  Biotehnologija  Raziskovalec  2021 - 2022  573 
22.  20842  dr. Aleksander Marinšek  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  348 
23.  24268  dr. Tanja Mrak  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  117 
24.  11279  dr. Nives Ogrinc  Varstvo okolja  Raziskovalec  2021 - 2022  1.035 
25.  51856  Sara Pintarič  Rastlinska produkcija in predelava  Mladi raziskovalec  2021 - 2022  10 
26.  17336  Matej Rupel    Tehnični sodelavec  2021 - 2022  71 
27.  10264  dr. Primož Simončič  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  678 
28.  25992  dr. Tomaž Skrbinšek  Biologija  Raziskovalec  2021 - 2022  204 
29.  52921  dr. Lidija Strojnik  Rastlinska produkcija in predelava  Raziskovalec  2021 - 2022  95 
30.  14056  dr. Marjetka Suhadolc  Rastlinska produkcija in predelava  Raziskovalec  2021 - 2022  226 
31.  31877  dr. Nataša Šibanc  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  72 
32.  17331  Magda Špenko    Tehnični sodelavec  2021 - 2022 
33.  54070  David Štefanič    Tehnični sodelavec  2021 - 2022 
34.  24777  Barbara Štupar    Raziskovalec  2021 - 2022  29 
35.  18510  dr. Martina Turk  Biokemija in molekularna biologija  Raziskovalec  2021 - 2022  173 
36.  39931  Rok Turniški  Rastlinska produkcija in predelava  Raziskovalec  2021 - 2022  42 
37.  38188  dr. Tina Unuk Nahberger  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  41 
38.  22592  dr. Urša Vilhar  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  383 
39.  21137  Daniel Žlindra  Gozdarstvo,  lesarstvo in papirništvo  Raziskovalec  2021 - 2022  157 
Organizacije (3)
št. Evidenčna št. Razisk. organizacija Kraj Matična številka Štev. publikacij
1.  0106  Institut "Jožef Stefan"  Ljubljana  5051606000  84.122 
2.  0404  Gozdarski inštitut Slovenije  Ljubljana  5051673000  11.663 
3.  0481  Univerza v Ljubljani, Biotehniška fakulteta  Ljubljana  1626914  63.768 
Povzetek
Soil biodiversity is the variation in soil life, from genes to communities, and the variation in soil habitats, from micro-aggregates to entire landscapes. The complexity and interdependence of soil habitats, biota diversity, and functional traits in soil are complex and poorly understood. Pre-DNA soil biodiversity assessments were limited by taxonomic knowledge of involved specialists. Nowadays by using a DNA-based approaches a representative soil sampling remains as one of main obstacles in representative soil biodiversity studies, which could be overcome with using leached water representing larger volume of soils. Soils have a water permeability/filtering ecosystem function. In its passage through soil layers water dilutes inorganic and organic elements, while its capacity for carrying eDNA remains largely unknown, despite the eDNA metabarcoding is and established approach for tracing organisms and biodiversity assessment in fresh and marine waters. An eDNA metabarcoding was also successful on bulk soils, but up to date, there is no published information on using of an eDNA approach in waters leached from soils. The proposed study aims to test four soil water related hypotheses for use of an eDNA as a soil biodiversity proxy, namely: (i) comes eDNA in water samples from lysimeters in a sufficient quantity and quality for a DNA metabarcoding-based soil biodiversity assessment; (ii) does eDNA in water samples gained from different lysimeter types yield a sufficient representation of soil communities of the target taxonomic group with a DNA metabarcoding, (iii) how distant from the lysimeter point the approach still reliably represent the soil biodiversity, and (iiii) how eDNA-assessed soil biota communities at selected taxonomic or functional levels from leached soil water change/differ with depth or soil horizons in forest, arable, and pastures land uses and if leached waters from different depths/horizons show dilution/selection/accumulation effects. Methodology approach will combine knowledge from water eDNA studies in aquatic systems, lysimeter soil water sampling approaches as used in permanent forest monitoring programmes (ICP Forests), and an eDNA metabarcoding of soil communities of archaea, bacteria, fungi, pedofauna, and plants. The eDNA metabarcoding will base on an adequate eDNA extraction from leached water followed by a sample multiplexing, and DNA metabarcoding. eDNA metabarcoding will include general markers for key soil organism kingdoms: archaea (16S rDNA), bacteria (16S rDNA), fungi (ITS rDNA), plants (rbcL or ITS2 rDNA) and animals/pedofauna (COI and optional Arth02 or Inse01 16S mt rDNA). Bioinformatics with general or adapted R scripts, and community statistics will be combined with key leached water and soil metadata (soil chemistry, isotopes, other environmental parameters) for explanation of community structure and for optimisation of sampling and eDNA treatment. The same methodology will be used in parallel with a total DNA extracted from soil cores as a reference for comparison to the eDNA samples. The experimental part of the project will end up in standardization of the final operating procedures, testing them and continuing methodological developments for specific soil types. The DNA barcoding data, supported by other relevant environmental data, will form a shared database, and will serve as the starting point for future monitoring works. The proposed operation procedure will be suggested as a monitoring approach to be included in exiting soil biodiversity programmes and reference procedures (such as ICP Forests). The national project partners team will continue networking with relevant national and international stakeholders for visibility and internationalisation of protocols (starting with the proposed international partners) and further development of specific ecosystem-adapted protocols.
Zgodovina ogledov
Priljubljeno