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Projects / Programmes source: ARIS

Inflammatory process in interstitial cystitis and evaluation of the influence of cannabinoid receptor agonists in urinary bladder - from cells to patients

Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
3.01  Medical and Health Sciences  Basic medicine 
Keywords
interstitial cystitis, inflammation, blood-urinary barrier, cannabinoids, tight junctions, cell signaling, urinary bladder
Evaluation (rules)
source: COBISS
Researchers (29)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  54920  PhD Uroš Cerkvenik  Biology  Researcher  2023  11 
2.  23503  Sanja Čabraja    Technical associate  2020 - 2022 
3.  34354  PhD Urška Dragin Jerman  Oncology  Researcher  2021  60 
4.  15873  PhD Mateja Erdani Kreft  Neurobiology  Researcher  2020 - 2023  407 
5.  23916  PhD Andreja Erman  Biochemistry and molecular biology  Researcher  2020 - 2023  117 
6.  09275  PhD Damjan Glavač  Chemistry  Researcher  2020 - 2023  561 
7.  25780  PhD Samo Hudoklin  Neurobiology  Researcher  2020 - 2023  108 
8.  20370  PhD Mojca Kerec Kos  Pharmacy  Researcher  2020 - 2023  238 
9.  00412  PhD Igor Križaj  Biochemistry and molecular biology  Researcher  2020 - 2023  715 
10.  39162  PhD Tadeja Kuret  Microbiology and immunology  Researcher  2020 - 2022  64 
11.  28845  PhD Katja Lakota  Microbiology and immunology  Researcher  2020 - 2023  233 
12.  18802  PhD Adrijana Leonardi  Biochemistry and molecular biology  Researcher  2020 - 2023  153 
13.  56647  Hristina Obradović  Neurobiology  Researcher  2022 
14.  17889  Nada Pavlica Dubarič    Technical associate  2020 - 2023 
15.  20213  PhD Toni Petan  Biochemistry and molecular biology  Researcher  2020 - 2023  177 
16.  01502  PhD Metka Ravnik-Glavač  Biochemistry and molecular biology  Researcher  2020 - 2023  268 
17.  28393  PhD Nataša Resnik  Biochemistry and molecular biology  Researcher  2020 - 2023  88 
18.  11654  PhD Rok Romih  Neurobiology  Researcher  2020 - 2023  248 
19.  11224  Igor Sterle    Technical associate  2022 - 2023  29 
20.  34712  PhD Mojca Stojan Dolar  Interdisciplinary research  Researcher  2020 - 2022  71 
21.  21553  PhD Jernej Šribar  Biochemistry and molecular biology  Researcher  2020 - 2023  108 
22.  17885  Linda Štrus    Technical associate  2020 - 2023 
23.  13601  PhD Matija Tomšič  Microbiology and immunology  Researcher  2020 - 2023  764 
24.  33100  PhD Larisa Tratnjek  Neurobiology  Researcher  2020 - 2023  61 
25.  08279  PhD Peter Veranič  Neurobiology  Head  2020 - 2023  359 
26.  20343  PhD Daša Zupančič  Biochemistry and molecular biology  Researcher  2020 - 2023  88 
27.  19588  Sabina Železnik    Technical associate  2020 - 2023 
28.  39124  PhD Taja Železnik Ramuta  Biochemistry and molecular biology  Researcher  2020 - 2021  103 
29.  56000  Mia Žganjar  Biochemistry and molecular biology  Researcher  2021 - 2023  10 
Organisations (4)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0106  Jožef Stefan Institute  Ljubljana  5051606000  90,038 
2.  0312  University Medical Centre Ljubljana  Ljubljana  5057272000  76,699 
3.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  47,545 
4.  0787  University of Ljubljana, Faculty of Pharmacy  Ljubljana  1626973  17,536 
Abstract
Interstitial cystitis (IC) is a urinary bladder disorder with no obvious histopathology or bacterial infection. Its prevalence is up to 2% in females, diagnosed annually, and its incidence is increasing globally. Since the etiology of IC is poorly understood, this illness still lacks an efficient cure, as the current treatment is still not successful in more than a third of the patient population. A general indicator of IC is an increase in inflammatory markers which is supposed to induce altered urothelial barrier function and abnormal urothelial sensory / signaling function that further induce inflammation, causing a vicious circle. However, the mechanism by which inflammation interacts with both defects is poorly understood. Studies of barrier components and receptors involved in urothelial signaling in healthy and diseased bladders lack a multidisciplinary approach, which would include epigenetic, proteomic and cell-biological approaches. This shortcoming leads to often contradictory and confusing conclusions. Such multilevel studies have not been done yet, mainly because of the technically demanding cryo-electron microscopic and proteomic methods required for that. Additionally, epigenetic studies that could explain why chronic symptoms, similar to those of bacterial cystitis, persist for years in women with IC without an obvious cause have not been used sufficiently in urothelial tissue. Since the exploration of non-psychogenic cannabinoids as anti-inflammatory treatment is exponentially growing recently, the aim of the present study is to determine whether selected cannabinoids can reduce inflammation of urinary bladder associated with interstitial cystitis and whether they can recover the barrier function of this tissue. Our multidisciplinary epigenetic, proteomic and cell biological analysis of urothelial cells collected from IC patients, experimental animals and biomimetic cell culture system will provide evidence for the potential of the cannabinoid treatment in case of IC . The ultimate goal of this study is to better understand the molecular mechanisms of urothelial hypersensitivity in chronic urinary bladder inflammation in IC. The results of this study have a realistic chance to provide the basis for development of new drugs leading to a better treatment of IC.
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