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Projects / Programmes source: ARIS

Rastlinska fiziologija in biotehnologija (Slovene)

Periods
Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   
1.03.00  Natural sciences and mathematics  Biology   

Code Science Field
B310  Biomedical sciences  Physiology of vascular plants 
T490  Technological sciences  Biotechnology 
B191  Biomedical sciences  Plant biochemistry 
B390  Biomedical sciences  Phytotechny, horticulture, crop protection, phytopathology 
Evaluation (rules)
source: COBISS
Researchers (20)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  19116  PhD Špela Baebler  Biotechnology  Researcher  2001 - 2003  313 
2.  18343  Aleš Blatnik    Researcher  2001 - 2003  49 
3.  22614  PhD Jana Boben  Biotechnology  Researcher  2003  82 
4.  21646  PhD Jernej Brzin  Biology  Researcher  2001 - 2003  46 
5.  08276  PhD Marjana Camloh  Biotechnology  Researcher  2001 - 2003  155 
6.  23607  PhD Katarina Cankar  Biotechnology  Researcher  2003  88 
7.  23606  PhD Matjaž Hren  Biology  Researcher  2003  114 
8.  04333  PhD Maja Kovač  Biotechnology  Researcher  2001 - 2003  204 
9.  19120  PhD Hana Krečič Stres  Biotechnology  Researcher  2001 - 2003  50 
10.  18344  Lidija Matičič    Researcher  2001 - 2003  42 
11.  15489  PhD Irena Mavrič Pleško  Plant production  Researcher  2001 - 2003  380 
12.  19119  PhD Mojca Milavec  Biotechnology  Researcher  2001 - 2003  318 
13.  20397  PhD Veronika Okršlar  Biochemistry and molecular biology  Researcher  2001 - 2003  22 
14.  10263  PhD Nataša Petrovič  Biotechnology  Researcher  2003  161 
15.  24505  PhD Manca Pirc  Biotechnology  Researcher  2003  210 
16.  18467  PhD Maruša Pompe Novak  Biotechnology  Researcher  2001 - 2003  291 
17.  05229  PhD Maja Ravnikar  Biotechnology  Head  2001 - 2003  1,369 
18.  18347  Marija Slovnik-Udovč    Researcher  2003 
19.  15878  PhD Darja Stanič  Biotechnology  Researcher  2001 - 2003  58 
20.  03765  PhD Jana Žel  Biotechnology  Researcher  2001 - 2003  619 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,239 
Abstract
The programme in Plant Physiology and Biotechnology is designed to bring basic research in the former to bear on studies for industrial applications. Research is centred on - responses of plants to stress caused by pathogens - transformation of plants for the development of virus resistant plants - identification and characterization of plant pathogens and development of diagnostic methods - production of secondary metabolites in tissue culture. Plant defence against pathogens is economically very important. The symptoms of infection are well documented but the physiological responses, and hence the natural mechanisms of resistance, are still not understood. We will study the metabolism of plant hormones, particularly jasmonates and salicylic acid, which function as signal molecules in the physiological response of plants to pathogens. As infection often resulted in chlorotic leaves, the metabolism of photosynthetic pigments and peroxidase activity will be also followed. The cellular location of proteins involved in defence, in plants stressed by jasmonic acid or virus infection, will be studied by immuno-localization using electron microscopy, to shed light on their function. The function of plant hormones (jasmonates, cytokinins and auxins) will be studied also in healthy plants. Resistance to pathogens will be introduced by transforming plants using molecular biology and plant tissue culture. The RNA level and DNA copies of introduced genes in already established transformed lines will be determined and the level of introduced resistance evaluated by infection tests. Genetically modified plants resistant to pathogens will be used in physiological studies of plant defence. Work on the identification and characterization of plant pathogens will be continued. This depends on the application and development of methods like electron microscopy, serological techniques, fatty acid profiles and molecular biology tools. Plants are potential factories for producing natural products such as drugs, if they can be grown in suitable types of tissue culture. Selected pharmaceutically important plants will be used to establish and assess different types of culture. Some will be transformed by Agrobacterium rhizogenes to establish hairy root cultures. This research will require the development of suitable methods for detecting secondary metabolites, such as HPLC, ELISA, spectrophotometry and electrophoresis.
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
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