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Projects / Programmes source: ARIS

Proteomics as a tool for monitoring biosynthesis processes

Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
Keywords
proteomics, 2-D electrophoresis, CIM monoliths, post-translational modifications, phosphorylation, glycosylation
Evaluation (rules)
source: COBISS
Researchers (10)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  12688  PhD Kristina Gruden  Biotechnology  Researcher  2005 - 2008  983 
2.  18511  PhD Polona Jamnik  Biotechnology  Researcher  2006 - 2008  903 
3.  28220  PhD Damjan Janeš  Pharmacy  Researcher  2007 - 2008  233 
4.  04648  PhD Janko Kos  Biotechnical sciences  Head  2005 - 2008  1,154 
5.  19120  PhD Hana Krečič Stres  Biotechnology  Researcher  2006  50 
6.  19119  PhD Mojca Milavec  Biotechnology  Researcher  2005 - 2008  318 
7.  27755  PhD Roman Paškulin  Biotechnology  Researcher  2006 - 2008  85 
8.  13542  PhD Hrvoje Petković  Biotechnology  Researcher  2005 - 2008  299 
9.  04001  PhD Peter Raspor  Microbiology and immunology  Researcher  2006 - 2008  1,895 
10.  03765  PhD Jana Žel  Biotechnology  Researcher  2005 - 2008  619 
Organisations (3)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,315 
2.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,549 
3.  0787  University of Ljubljana, Faculty of Pharmacy  Ljubljana  1626973  17,522 
Abstract
Proteomics is becoming an important scientific discipline. It has advantages that cannot be sustained with procedures of genomics and transcriptomics. It studies proteins that are key actors of functions in all living cells. We will focused on introduction of proteomics with the help of 2-D electrophoresis, which is one of the most widely used method in proteomics for protein separation. Firstly the aim will be develop and optimize 2-D electrophoresis to study total protein expression on selected model organisms. Model organisms such as animal cell line, bacteria Streptomyces rimosus and potato Solanum tuberosum, that have already been research subject of the research groups involved in the project, will be included. For each selected model organism, the key points of particular 2-D electrophoresis steps, such as sample preparation, pH gradient selection, gel percentage selection, staining technique will be optimized. Furthermore, 2-D electrophoresis has ability to detect post-translational modifications, which cannot be predicted from the genome sequence. Knowledge of post-translational modifications facilitates insights into biological events. Therefore, a procedure for detection of phosphoproteins and glycoproteins will be optimized In continuation of the project we will test the application of proteomics as a tool for monitoring biosynthesis processes such as biosynthesis of homologous and heterologous proteins. Different model cases will be included. Finally, the manual (collection of protocols) will be prepared. It will provide optimized procedures with troubleshooting experimentally determined for particular model organisms. This will help different research groups to avoid difficulties and to spare time and money at their future work.
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