Projects / Programmes source: ARRS

Transkriptom plodu oljke ? razvoj oznak izraženih zaporedij (EST) oljčnega plodu za študije tkivno specifičnih metabolnih poti (Slovene)

Research activity

Code Science Field Subfield
4.03.01  Biotechnical sciences  Plant production  Agricultural plants 

Code Science Field
4.04  Agricultural and Veterinary Sciences  Agricultural biotechnology 
Evaluation (rules)
source: COBISS
Researchers (10)
no. Code Name and surname Research area Role Period No. of publications
1.  20038  PhD Dunja Bandelj  Biotechnology  Researcher  2009 - 2012  264 
2.  28496  PhD Tjaša Gril  Plant production  Researcher  2009  18 
3.  30863  PhD Matjaž Hladnik  Biotechnical sciences  Junior researcher  2009 - 2012  80 
4.  16379  PhD Jernej Jakše  Plant production  Principal Researcher  2009 - 2012  629 
5.  05994  PhD Branka Javornik  Plant production  Researcher  2009 - 2012  1,282 
6.  09211  PhD Boris Kryštufek  Biology  Researcher  2009 - 2012  950 
7.  30862  PhD Tjaša Marolt  Chemistry  Junior researcher  2009 - 2012  10 
8.  27613  PhD Maja Podgornik  Plant production  Researcher  2009 - 2012  179 
9.  15577  Nevenka Valič  Plant production  Researcher  2010 - 2012  89 
10.  17107  PhD Irma Vuk  Biotechnical sciences  Researcher  2009 - 2012  90 
Organisations (2)
no. Code Research organisation City Registration number No. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  63,774 
2.  1510  Science and Research Centre Koper  Koper  7187416000  11,341 
Significance for science
Olive is one of the oldest cultivated fruit trees and oil producing crops of the Mediterranean basin, with very high historical, cultural and economic relevance. It also plays a fundamental role in the landscape maintenance of some regions. Olive production is globally the most important agricultural branch of the aforementioned region and olive oil is the principal source of fats in the rich Mediterranean diet. This study on EST development and characterization from developing olive fruit using next generation sequencing technologies (NGS) has enabled us to generate the first larger amount of nucleotide data for olive. They are all accessible through the NCBI SRA archive. The project results are also of interest to international collaborators who are engaged in olive research. In collaboration with a French research group from INRA Montpellier, genic microsatellite markers were developed for use in genotyping and linkage analysis studies. The project results have enabled us to characterize a large number of genes used for normalization of RT-qPCR studies and to gain an insight into the expression of selected genes that are involved in the synthesis of fatty acids in developing olive fruit. The results have also contributed to new knowledge about the biochemical characteristics of the primary metabolism (synthesis of fatty acids) of olive fruit. The knowledge gained will also contribute to fundamental knowledge of other biological processes in olive fruit. Secondary metabolism of biophenols is very important in relation to superior olive oil storage capacity and from a pharmaceutical point of view. Annotation of transcripts yielded 924 sequences that are putatively involved in the processes of secondary metabolism. The basic results achieved will have a broad impact on the development of applications and science in this field.
Significance for Slovenia
The research results have contributed to the acquisition of new knowledge in Slovenia and, consequently, to incorporating such knowledge into new technologies both in Slovenia and in the wider world. We have successfully gained the necessary bioinformatic skills for manipulation and analysis of NGS data. This project, together with other projects of the Biotechnical Faculty group, has made an important contribution to the development of the bioinformatics field – we have decided to develop a powerful server class computer with dedicated software for NGS data analysis. Vegetable oils provide approximately 25% of the calories consumed by industrial nations. In addition to their dietary significance, vegetable oils are a major agricultural commodity, with worldwide production of 40 billion kilograms, worth nearly 35 billion € per year. This large market and the fact that the fatty acid composition of vegetable oils influences both their physical properties and nutritional characteristics, has encouraged considerable interest in modifying plant fatty acid production and synthesis of pharmacologically active molecules, including biophenols and vitamins. Genomic approaches, including EST sequencing, are now contributing to greater understanding of the underlying metabolism of oil plants and the regulatory networks that determine the quality and quantity of oils produced. The project has also been important for the education of human resources. A young researcher has been trained within the context of the project tasks and she has successfully gained knowledge and the necessary bioinformatics skills. In Slovenia, olive is second to apple in terms of the area used for its cultivation. Slovenian olive oil was the first Slovene agricultural product with a quality mark certified by the European Union. This research proposal also has indirect significance for society in terms of its potential for educating the young. Education is crucial for developing innovation and for transferring research findings into the economy. The project results are also an ideal learning platform for undergraduate and graduate students for learning the skills of NGS analysis.
Most important scientific results Annual report 2009, final report, complete report on dLib.si
Most important socioeconomically and culturally relevant results Annual report 2011, final report, complete report on dLib.si
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