Projects / Programmes
January 1, 1999
- December 31, 2003
Code |
Science |
Field |
Subfield |
4.06.00 |
Biotechnical sciences |
Biotechnology |
|
4.02.00 |
Biotechnical sciences |
Animal production |
|
Code |
Science |
Field |
P300 |
Natural sciences and mathematics |
Analytical chemistry |
P310 |
Natural sciences and mathematics |
Proteins, enzymology |
P340 |
Natural sciences and mathematics |
Lipids, steroids, membranes |
B230 |
Biomedical sciences |
Microbiology, bacteriology, virology, mycology |
B680 |
Biomedical sciences |
Public health, epidemiology |
T130 |
Technological sciences |
Production technology |
Researchers (18)
Organisations (1)
Abstract
A lot of different research work, mainly focused on studying the chemistry, enzymology and microbiology of milk and dairy products is included in the research program. Mechanisms of protein and fat degradation and biosynthesis of components contributing to formation of aromatic, rheorogical and nutritional characteristic of dairy products are investigated. The potentially pathogenic bacteria with their negative effects on milk and dairy products will be analysed as well. Species of Bacillus genera are of special interest in numerous investigations lately, because they form spores and some strains are even potential pathogens. Earlier researches revealed that in particular seasons of the year 69 up to 81 % of raw milk samples were contaminated with Bacillus cereus species, while as much as 83 up to 94 % among all of isolates from the milk or milk products showed production of the diarrheal toxin. Our main task now is classification of these isolates according to their homology of the specific DNA regions.
The second field of research in our laboratory is pointed in examination of lactic acid bacteria as protective starter cultures, as host cells for bacteriophages and as probiotics. In our collection we posses some very interesting bacteriocinogenic isolates, of which mechanisms of bacteriocin synthesis, antibacterial activity, survival in diverse microbial populations and ability to colonise the intestine are studied. The synthesis of bacteriocins encompasses four basic genes, organised in one or two operons. These genes are: structural gene encoding prebacteriocin, immunity gene, gene encoding an ABC-transporter and gene encoding an accessory protein. The whole genetic structure of bacteriocin is analysed by preparation of the gene library, where only transformants active against indicator strain are selected for further examinations. Hybridisation techniques, in which the bacteriocin DNA specific probe is employed, help to detect the survival of the bacteriocinogenic strains in the intestine of lab animals or in fermented food. Moreover, the potential use of immunological methods as strain tracking mode is studying as well.
Most important scientific results
Final report
Most important socioeconomically and culturally relevant results
Final report