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Projects / Programmes source: ARRS

Milk and dairy products

Periods
Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   
4.02.00  Biotechnical sciences  Animal production   

Code Science Field
P300  Natural sciences and mathematics  Analytical chemistry 
P310  Natural sciences and mathematics  Proteins, enzymology 
P340  Natural sciences and mathematics  Lipids, steroids, membranes 
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
B680  Biomedical sciences  Public health, epidemiology 
T130  Technological sciences  Production technology 
Evaluation (rules)
source: COBISS
Researchers (18)
no. Code Name and surname Research area Role Period No. of publications
1.  17522  Marta Berlec    Researcher  2002 - 2003 
2.  11150  PhD Bojana Bogovič Matijašić  Animal production  Researcher  2002 - 2003  375 
3.  15659  PhD Andreja Čanžek Majhenič  Animal production  Researcher  2002 - 2003  232 
4.  17523  Viktor Gašparič    Researcher  2002 - 2003 
5.  05099  PhD Karmen Godič Torkar  Microbiology and immunology  Researcher  2002 - 2003  256 
6.  01864  PhD Stanislava Golc Teger  Animal production  Researcher  2002 - 2003  153 
7.  17524  Pavle Jeras    Researcher  2002 - 2003 
8.  17525  Irena Jereb    Researcher  2002 - 2003 
9.  01863  Dušan Kastelic  Animal production  Researcher  2002 - 2003  43 
10.  17526  Andrej Kolenc    Researcher  2002 - 2003 
11.  19330  PhD Valentina Lenasi  Animal production  Researcher  2002 - 2003  38 
12.  15141  PhD Andreja Miklič  Animal production  Researcher  2002 - 2003  35 
13.  03135  Marko Miklič  Animal production  Researcher  2002 - 2003  24 
14.  06375  Vanja Penca Habjan  Animal production  Researcher  2002 - 2003  16 
15.  06981  PhD Bogdan Perko  Animal production  Researcher  2002 - 2003  216 
16.  20456  Stanislava Podkrajšek    Researcher  2002 - 2003  12 
17.  08857  PhD Irena Rogelj  Animal production  Principal Researcher  2002 - 2003  706 
18.  21439  PhD Saša Stojković  Biotechnology  Researcher  2002 - 2003  15 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  63,852 
Abstract
A lot of different research work, mainly focused on studying the chemistry, enzymology and microbiology of milk and dairy products is included in the research program. Mechanisms of protein and fat degradation and biosynthesis of components contributing to formation of aromatic, rheorogical and nutritional characteristic of dairy products are investigated. The potentially pathogenic bacteria with their negative effects on milk and dairy products will be analysed as well. Species of Bacillus genera are of special interest in numerous investigations lately, because they form spores and some strains are even potential pathogens. Earlier researches revealed that in particular seasons of the year 69 up to 81 % of raw milk samples were contaminated with Bacillus cereus species, while as much as 83 up to 94 % among all of isolates from the milk or milk products showed production of the diarrheal toxin. Our main task now is classification of these isolates according to their homology of the specific DNA regions. The second field of research in our laboratory is pointed in examination of lactic acid bacteria as protective starter cultures, as host cells for bacteriophages and as probiotics. In our collection we posses some very interesting bacteriocinogenic isolates, of which mechanisms of bacteriocin synthesis, antibacterial activity, survival in diverse microbial populations and ability to colonise the intestine are studied. The synthesis of bacteriocins encompasses four basic genes, organised in one or two operons. These genes are: structural gene encoding prebacteriocin, immunity gene, gene encoding an ABC-transporter and gene encoding an accessory protein. The whole genetic structure of bacteriocin is analysed by preparation of the gene library, where only transformants active against indicator strain are selected for further examinations. Hybridisation techniques, in which the bacteriocin DNA specific probe is employed, help to detect the survival of the bacteriocinogenic strains in the intestine of lab animals or in fermented food. Moreover, the potential use of immunological methods as strain tracking mode is studying as well.
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Most important socioeconomically and culturally relevant results Final report
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