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Projects / Programmes source: ARIS

Molecular basis of tolerance to abiotic stress in Phaseolus sp.

Research activity

Code Science Field Subfield
4.03.00  Biotechnical sciences  Plant production   

Code Science Field
B191  Biomedical sciences  Plant biochemistry 
B225  Biomedical sciences  Plant genetics 
B420  Biomedical sciences  Nutrition 
Keywords
plant stress, drought, flooding, differentially expressed genes, proteolytic enzymes, common bean, Phaseolus vulgaris L.,DD-RT-PCR, DNA microarray
Evaluation (rules)
source: COBISS
Researchers (14)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  26491  PhD Maruška Budič  Biochemistry and molecular biology  Junior researcher  2006 - 2007  24 
2.  12688  PhD Kristina Gruden  Biotechnology  Researcher  2006 - 2007  981 
3.  18981  PhD Tatjana Kavar  Animal production  Researcher  2005 - 2007  75 
4.  14804  PhD Marjetka Kidrič  Biochemistry and molecular biology  Researcher  2004 - 2007  118 
5.  04648  PhD Janko Kos  Biotechnical sciences  Researcher  2006 - 2007  1,154 
6.  22937  Marija Kregar    Technical associate  2004 - 2007 
7.  23514  PhD Marko Maras  Plant production  Junior researcher  2004 - 2007  118 
8.  05667  PhD Vladimir Meglič  Plant production  Head  2004 - 2007  860 
9.  21551  PhD Nina Slapar  Pharmacy  Researcher  2006  21 
10.  15658  PhD Simona Sušnik Bajec  Biochemistry and molecular biology  Researcher  2004  194 
11.  07849  PhD Borut Štrukelj  Biochemistry and molecular biology  Researcher  2006 - 2007  1,120 
12.  08500  PhD Jelka Šuštar Vozlič  Plant production  Researcher  2004 - 2007  500 
13.  16393  PhD Kristina Ugrinović  Plant production  Researcher  2004 - 2007  638 
14.  17285  Darja Žunič Kotar    Technical associate  2007 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0106  Jožef Stefan Institute  Ljubljana  5051606000  89,961 
2.  0401  Agricultural institute of Slovenia  Ljubljana  5055431  20,095 
Abstract
Water stress is the cause of important damage to plants at the cellular level, and responsible for substantial yield loss throughout the world. Research on the physiological and biochemical basis of the plant"s response is necessary for understanding plant growth and development, and for crop improvement. The aim of this project is to study the response of several bean species and common bean cultivars with different sensitivities to drought and flooding, in order to evaluate the molecular basis of greater resistance to stress. On the basis of monitoring several physiological parameters, appropriate plants will be selected. Analysis of their gene expression by differential display RT-PCR and quantitative real-time PCR will be carried out. In order to follow a large number of genes at a time a DNA microarrays methodology will be initiated. Differentially expressed genes in leaves of drought stressed and control plants will be analysed. Their expression in different cultivars and under flooding stress will be determined. Additionally, changes in activity of proteolytical enzymes affecting selective or uncontrolled protein breakdown will be studied. The scanning procedure developed in our laboratory will be used to identify the proteases and/or their endogenous inhibitors, and to assign individual proteases to an identified change in activity under stress. The activities of proteases in control and stressed plants with different sensitivities will then be compared on a quantitative basis. Selected protease(s) will be characterised, in order to perform cDNA cloning and expression analysis. The proposed investigation will contribute to a deeper understanding of the mechanism of plant response to drought and flooding and, ultimately, to improving resistance in Phaseolus cultivars. It will also enable us to join international efforts already initiated to improve the quality and yield of common bean.
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