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Projects / Programmes source: ARIS

Mikrobna biotehnologija in mikrobiologija (Slovene)

Periods
Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
Evaluation (rules)
source: COBISS
Researchers (17)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  17799  Ivana Avbelj    Researcher  2002 - 2003 
2.  07469  PhD Martin Batič  Biotechnology  Researcher  2002 - 2003  193 
3.  18332  PhD Neža Čadež  Biotechnology  Researcher  2002 - 2003  322 
4.  21392  PhD Štefan Fujs  Biotechnology  Researcher  2002 - 2003  87 
5.  18511  PhD Polona Jamnik  Biotechnology  Researcher  2002 - 2003  904 
6.  21462  PhD Špelca Jenko  Pharmacy  Researcher  2002 - 2003  18 
7.  22661  MSc Sergej Jerman  Plant production  Researcher  2003  14 
8.  11548  PhD Barbka Jeršek  Animal production  Researcher  2002 - 2003  419 
9.  22491  PhD Anja Klančnik  Animal production  Researcher  2003  387 
10.  22452  PhD Damjana Miklič Milek  Biotechnology  Researcher  2003  152 
11.  18126  PhD Katja Povhe Jemec  Biotechnology  Researcher  2002 - 2003  61 
12.  04001  PhD Peter Raspor  Microbiology and immunology  Head  2002 - 2003  1,898 
13.  14141  Marjeta Recek  Biotechnology  Researcher  2002 - 2003  60 
14.  07030  PhD Sonja Smole - Možina  Animal production  Researcher  2002 - 2003  1,104 
15.  19506  Matej Šergan    Researcher  2002 - 2003 
16.  15874  PhD Tatjana Zagorc  Interdisciplinary research  Researcher  2002 - 2003  62 
17.  20705  PhD Tina Zorman  Biotechnology  Researcher  2003  66 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,306 
Abstract
In the past year the research group of Microbial Biotechnology and Microbiology was active in the focused field of research program. The identification system in combination with the molecular-biological and classical techniques for yeast determination was placed. The identification system was tested on the yeasts isolated from the traditional fermentation of kefir – 107 isolates were identified and characterised (COBISS-ID 2326395; 2326648), among them 12 isolates were deposit in the culture collection. Among the yeast isolated from the malvasiya must 912 isolates were determined and characterised (COBISS-ID 2259064), and 80 isolates was deposit in culture collection. The product of six spontaneous and nine induced wine fermentations was characterised by chemical, microbiological and sensorical analysis. The 2286 isolates were analysed including the tests of killer activity. The electrophoretic karyotypes of 1486 isolates of Saccharomyces and other wine yeasts were determined. Further more the isolates of acetic-acid bacteria from the vinegar was identified and characterised (COBIDSS-ID 2282360; 2320248). Bioaccumulation of metallic ions with microorganisms include investigation of environmental effect as well as intrinsic biochemical, structural and genetic characteristic of yeast cells during process of bioaccumulation. Environmental factors such as concentrations of metal ions, pH, temperature, concentration of oxygen, etc. influence processes of metal translocation metal speciation in the cell. In the chromium case was demonstrated that different concentrations of Cr3+ compounds (0.1 to 10 mM) effected yeast physiology and chromium accumulation in yeast C. intermedia (COBISS-ID 2388600, COBISS-ID2284152). Growth phase influenced chromium biosorption and accumulation (COBISS-ID 2329720, COBISS-ID 2416248, COBISS-ID 2417016) and using EPR spectroscopy the effect of chromium on yeast membrane permeability was confirm (COBISS-ID 2301048). Meanwhile the mathematical model for yeasts viability/mortality screening tests among different Cr3+or6+ was build up (COBISS-ID 2282104; 2295160). Model mediate basic information in tolerance between yeasts. The achieved results of Microbial Biotechnology and Microbiology research group build the main foundation of scientific review article on this field (COBISS-ID2388600). The research group focused on the microbial characterisation of the microorganisms with the negative activity as inducer of infection and food spoilage. Group identified the authenticity of e.g. Salmonella and settled the method for typing of phatogenic microorganisms e.g. Listeria monocytogenes in Bacillus cereus. The PCR techniques for strain Salmonella determination in foods were applied. Comparatively the classical methods according to ISO standards (ISO 6579, 1993) were used for the same bacterial strain identification. The results showed that a PCR method is faster compared to the classical method with the same reliability and sensitivity (COBISS-ID 2302584). For strains of L. monocytogenes the rep-PCR was performed for typing. A specific oligonucleotide pair for REP and ERIC starting point for L. monocytogenes typing from human, animal and food isolates was evaluated.
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
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