Projects / Programmes
Mikrobna biotehnologija in mikrobiologija (Slovene)
January 1, 1999
- December 31, 2003
| Code |
Science |
Field |
Subfield |
| 4.06.00 |
Biotechnical sciences |
Biotechnology |
|
| Code |
Science |
Field |
| T490 |
Technological sciences |
Biotechnology |
| B230 |
Biomedical sciences |
Microbiology, bacteriology, virology, mycology |
Researchers (17)
Organisations (1)
Abstract
In the past year the research group of Microbial Biotechnology and Microbiology was active in the focused field of research program. The identification system in combination with the molecular-biological and classical techniques for yeast determination was placed. The identification system was tested on the yeasts isolated from the traditional fermentation of kefir – 107 isolates were identified and characterised (COBISS-ID 2326395; 2326648), among them 12 isolates were deposit in the culture collection. Among the yeast isolated from the malvasiya must 912 isolates were determined and characterised (COBISS-ID 2259064), and 80 isolates was deposit in culture collection. The product of six spontaneous and nine induced wine fermentations was characterised by chemical, microbiological and sensorical analysis. The 2286 isolates were analysed including the tests of killer activity. The electrophoretic karyotypes of 1486 isolates of Saccharomyces and other wine yeasts were determined. Further more the isolates of acetic-acid bacteria from the vinegar was identified and characterised (COBIDSS-ID 2282360; 2320248).
Bioaccumulation of metallic ions with microorganisms include investigation of environmental effect as well as intrinsic biochemical, structural and genetic characteristic of yeast cells during process of bioaccumulation. Environmental factors such as concentrations of metal ions, pH, temperature, concentration of oxygen, etc. influence processes of metal translocation metal speciation in the cell. In the chromium case was demonstrated that different concentrations of Cr3+ compounds (0.1 to 10 mM) effected yeast physiology and chromium accumulation in yeast C. intermedia (COBISS-ID 2388600, COBISS-ID2284152). Growth phase influenced chromium biosorption and accumulation (COBISS-ID 2329720, COBISS-ID 2416248, COBISS-ID 2417016) and using EPR spectroscopy the effect of chromium on yeast membrane permeability was confirm (COBISS-ID 2301048). Meanwhile the mathematical model for yeasts viability/mortality screening tests among different Cr3+or6+ was build up (COBISS-ID 2282104; 2295160). Model mediate basic information in tolerance between yeasts. The achieved results of Microbial Biotechnology and Microbiology research group build the main foundation of scientific review article on this field (COBISS-ID2388600).
The research group focused on the microbial characterisation of the microorganisms with the negative activity as inducer of infection and food spoilage. Group identified the authenticity of e.g. Salmonella and settled the method for typing of phatogenic microorganisms e.g. Listeria monocytogenes in Bacillus cereus. The PCR techniques for strain Salmonella determination in foods were applied. Comparatively the classical methods according to ISO standards (ISO 6579, 1993) were used for the same bacterial strain identification. The results showed that a PCR method is faster compared to the classical method with the same reliability and sensitivity (COBISS-ID 2302584). For strains of L. monocytogenes the rep-PCR was performed for typing. A specific oligonucleotide pair for REP and ERIC starting point for L. monocytogenes typing from human, animal and food isolates was evaluated.
Most important scientific results
Final report
Most important socioeconomically and culturally relevant results
Final report
